Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Oct:9:100-103.
doi: 10.1016/j.redox.2016.07.003. Epub 2016 Jul 8.

Diabetes-induced oxidative stress in the vitreous humor

Zsuzsanna Géhl  1 , Edina Bakondi  2 , Miklós D Resch  1 , Csaba Hegedűs  2 , Katalin Kovács  3 , Petra Lakatos  2 , Antal Szabó  1 , Zoltán Nagy  1 , László Virág  4
Affiliations

Diabetes-induced oxidative stress in the vitreous humor

Zsuzsanna Géhl et al. Redox Biol. 2016 Oct.

Abstract

Purpose: Diabetes is accompanied by fundamental rearrangements in redox homeostasis. Hyperglycemia triggers the production of reactive oxygen and nitrogen species which contributes to tissue damage in various target organs. Proliferative diabetic retinopathy (PDR) is a common manifestation of diabetic complications but information on the possible role of reactive intermediates in this condition with special regard to the involvement of the vitreous in PDR-associated redox alterations is scarce. The aim of the study was to determine key parameters of redox homeostasis [advanced glycation endproducts (AGE); protein carbonyl and glutathione (GSH)] content in the vitreous in PDR patients.

Methods: The study population involved 10 diabetic patients undergoing surgery for complications of proliferative diabetic retinopathy and 8 control (non-diabetic) patients who were undergoing surgery for epiretinal membranes. Vitreal fluids were assayed for the above biochemical parameters.

Results: We found elevated levels of AGE in the vitreous of PDR patients (812.10 vs 491.69ng AGE/mg protein). Extent of protein carbonylation was also higher in the samples of diabetic patients (2.08 vs 0.67A/100μg protein). The GSH content also increased in the vitreous of PDR patients as compared to the control group (4.54 vs 2.35μmol/μg protein), respectively.

Conclusion: The study demonstrates that diabetes-associated redox alterations also reach the vitreous with the most prominent changes being increased protein carbonylation and increased antioxidant levels.

Keywords: Advanced glycation endproducts; Antioxidants; Diabetes; Glutathion; Protein carbonylation; Protein oxidation; Vitreous.

PubMed Disclaimer

Figures

fx1
Graphical abstract
Fig. 1.
Fig. 1
Vitreal samples were collected from diabetic and control patients and advanced glycation endproducts (AGE) (A), protein carbonylation (B) and reduced glutathione (GSH) content (C) have been determined as described in the Methods section. Star signs indicate significant difference between groups (⁎p<0.05, Mann-Whitney U-Test).
See this image and copyright information in PMC

References

    1. World Health Organization G. Global status report on noncommunicable diseases, 2014.
    1. World Health Organization G. Global health estimates: Deaths by cause, age, sex and country, 2014, 2000–2012.
    1. World Health Organization G. Causes Of blindness and visual impairment, 2016.
    1. (NEI) NEI. Facts about diabetic eye disease, 2015.
    1. Dobree J.H. Proliferative diabetic retinopathy: evolution of the retinal lesions. Br. J. Ophthalmol. 1964;48:637–649. - PMC - PubMed

Publication types