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. 1989 May;27(5):1112-4.
doi: 10.1128/jcm.27.5.1112-1114.1989.

Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever

F A Rubin  1 , P D McWhirter, N H Punjabi, E Lane, P Sudarmono, S P Pulungsih, M Lesmana, S Kumala, D J Kopecko, S L Hoffman
Affiliations

Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever

F A Rubin et al. J Clin Microbiol. 1989 May.

Abstract

A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.

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