Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Oct;23(10):755-762.
doi: 10.1007/s11655-016-2094-5. Epub 2016 Jul 26.

Inhibitory effect of the extract of Phellodendron amurense ruprecht root on collagen-induced arthritis in mice

Hyo Won Jung  1   2 , Kyung-Ho Kim  3 , Yong-Ki Park  4   5
Affiliations

Inhibitory effect of the extract of Phellodendron amurense ruprecht root on collagen-induced arthritis in mice

Hyo Won Jung et al. Chin J Integr Med. 2017 Oct.

Abstract

Objective: To investigate whether the dried root of Phellodendron amurense Ruprecht (Phellodendri cortex; PC) extract improves arthritic symptoms through anti-inflammatory and immune-modulatory effects in collagen-induced arthritis in mice.

Methods: Rheumatoid arthritis (RA) was induced in male DBA/1 mice by immunization with type II collagen (ColII). CIA mice were divided into 5 groups (n=10 per a group) with normal, CIA control, PC extract (50 mg/kg and 100 mg/kg)-treated, and meloxicam (50 mg/kg)-treated as the reference drug. The PC extract or meloxicam were administered orally in CIA mice once a day for 14 days after arthritis induction. Arthritic score, levels of anti-ColII IgG2a antibody, prostaglandin E2 (PGE2), tumor necrosis factor (TNF)-α, and interleukin (IL)-17 in the sera of CIA mice were measured. Histopathological changes in the ankle joints of CIA mice were also analyzed by staining with hematoxylin and eosin (H and E), safranin-O and immunohistochemistry using anti-TNF-α and anti-IL-17 antibodies.

Results: The arthritic score was increased in CIA mice in a time-dependent manner, as were the serum levels of anti-ColII IgG2a antibody, PGE2, TNF-α, and IL-17. However, the oral administration of PC extract at 50 and 100 mg/kg in CIA mice significantly decreased the arthritic scores, and the serum levels of anti-ColII IgG2a, PGE2, TNF-α, and IL-17 compared with those in the CIA group (P<0.05 or P<0.01). Furthermore, histopathological improvement of the joint architecture in CIA mice was observed after administration of PC extract. PC extract also significantly inhibited the expression of TNF-α and IL-17 in the joints of CIA mice by suppressing the expression of their mRNA and proteins.

Conclusion: PC extract may improve the pathological progression of RA through the inhibition of joint destruction by synovial inflammation and immune-stimulation, therefore, it would be a potential anti-arthritic agent in RA.

Keywords: Phellodendri cortex; Phellodendron amurense; anti-arthritic effect; collagen-induced arthritis.

PubMed Disclaimer

References

    1. Glycoconj J. 1999 Mar;16(3):247-52 - PubMed
    1. J Ethnopharmacol. 2011 Oct 11;137(3):1425-30 - PubMed
    1. J Interferon Cytokine Res. 2011 Dec;31(12):927-40 - PubMed
    1. Springer Semin Immunopathol. 2003 Aug;25(1):3-18 - PubMed
    1. Bioorg Med Chem. 2011 Jan 1;19(1):21-9 - PubMed

LinkOut - more resources