Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jul 1;170(1):321-6.
doi: 10.1084/jem.170.1.321.

Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line

J S Economou  1 , K Rhoades, R Essner, W H McBride, J C Gasson, D L Morton
Affiliations

Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line

J S Economou et al. J Exp Med. .

Abstract

The 615-bp 5' flanking region of the human TNF-alpha/cachectin gene was isolated and ligated to the luciferase reporter gene. In addition, a series of truncated promoter constructs was generated by exonuclease III digestion. The promoter activity of these constructs was studied in a transient transfection system using the TNF-alpha-producing U937 cell line. Full-length and truncated TNF promoter constructions extending from -615 to -95 bp relative to the transcription start site (TSS) could be induced by phorbol esters. A construct truncated to within 36 bp of the TSS (and within 11 bp of the TATAA box) was inactive. Therefore, the phorbol ester responsive is localized in the TNF/cachectin promoter to a relatively short region proximal to the TATAA box.

PubMed Disclaimer

References

    1. Science. 1985 Nov 8;230(4726):630-2 - PubMed
    1. Nucleic Acids Res. 1985 Sep 11;13(17):6361-73 - PubMed
    1. J Exp Med. 1986 Dec 1;164(6):2113-8 - PubMed
    1. Mol Cell Biol. 1987 Feb;7(2):725-37 - PubMed
    1. Cell. 1987 Oct 23;51(2):251-60 - PubMed

Publication types