Fluorometric high performance liquid chromatographic analysis of prostaglandin (PG) metabolites: application to the catabolism of PGE2 and PGF2 alpha in rat and rabbit kidney homogenates

Abstract

A previously reported fluorometric high performance liquid chromatographic procedure for separation of 9-anthryldiazomethane (ADAM) derivatives of prostaglandins (PG's) was applied to examine catabolic products of PG's, such as 15-keto-PGE2, 13,14-dihydro-15-keto-PGE2, 15-keto-PGF2 alpha, and 13,14-dihydro-15-keto-PGF2 alpha. ADAM derivatives of these metabolites were separated in 30 min on an ODS column with 65% acetonitrile. By this method catabolism of PGE2 and PGF2 alpha in kidney homogenates of rats and rabbits was analyzed. Homogenates of medulla and cortex of rat kidney catabolized PGE2 and PGF2 alpha to 15-keto-PG's. Whereas the homogenate of rabbit kidney cortex yielded 13,14-dihydro-15-keto-PGE2, that of medulla did not. The conversion of PGF2 alpha to PGE2 was shown to occur in homogenates of rabbit kidney cortex as well as rat kidney medulla and cortex.