Chemical Screening in Zebrafish

Colleen A Brady^{1

2

3}, Andrew J Rennekamp^{1

2

3}, Randall T Peterson^{4

5

6}

Affiliations

¹ Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA.
² Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA.
³ Broad Institute, 7 Cambridge Center, Cambridge, MA, 02142, USA.
⁴ Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA. PETERSON@CVRC.MGH.HARVARD.EDU.
⁵ Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA. PETERSON@CVRC.MGH.HARVARD.EDU.
⁶ Broad Institute, 7 Cambridge Center, Cambridge, MA, 02142, USA. PETERSON@CVRC.MGH.HARVARD.EDU.

PMID: 27464797
DOI: 10.1007/978-1-4939-3771-4_1

Chemical Screening in Zebrafish

Colleen A Brady et al. Methods Mol Biol. 2016.

. 2016:1451:3-16.

doi: 10.1007/978-1-4939-3771-4_1.

Authors

Colleen A Brady^{1

2

3}, Andrew J Rennekamp^{1

2

3}, Randall T Peterson^{4

5

6}

Affiliations

¹ Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA.
² Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA.
³ Broad Institute, 7 Cambridge Center, Cambridge, MA, 02142, USA.
⁴ Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA. PETERSON@CVRC.MGH.HARVARD.EDU.
⁵ Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA. PETERSON@CVRC.MGH.HARVARD.EDU.
⁶ Broad Institute, 7 Cambridge Center, Cambridge, MA, 02142, USA. PETERSON@CVRC.MGH.HARVARD.EDU.

PMID: 27464797
DOI: 10.1007/978-1-4939-3771-4_1

Abstract

Phenotypic small molecule screens in zebrafish have gained popularity as an unbiased approach to probe biological processes. In this chapter we outline basic methods for performing chemical screens with larval zebrafish including breeding large numbers of embryos, plating larval fish into multi-well dishes, and adding small molecules to these wells. We also highlight important considerations when designing and interpreting the results of a phenotypic screen and possible follow-up approaches, including popular methods used to identify the mechanism of action of a chemical compound.

Keywords: Multi-well plates; Phenotypic screening; Small molecules.

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Chemical Screening in Zebrafish

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Chemical Screening in Zebrafish

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