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. 2016 Jul 28;4(4):e00746-16.
doi: 10.1128/genomeA.00746-16.

Draft Genome Sequence of Agrobacterium rhizogenes Strain NCPPB2659

Jose A Valdes Franco  1 , Ray Collier  1 , Yi Wang  2 , Naxin Huo  2 , Yong Gu  1 , Roger Thilmony  3 , James G Thomson  3
Affiliations

Draft Genome Sequence of Agrobacterium rhizogenes Strain NCPPB2659

Jose A Valdes Franco et al. Genome Announc. .

Abstract

This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 (also known as strain K599). The assembled genome contains 5,277,347 bp, composed of one circular chromosome, the pRi2659 virulence plasmid, and 17 scaffolds pertaining to the linear chromosome. The wild-type strain causes hairy root disease in dicots and has been used to make transgenic hairy root cultures and composite plants (nontransgenic shoots with transgenic roots). Disarmed variants of the strain have been used to produce stable transgenic monocot and dicot plants.

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References

    1. Riker AJ, Banfield WM, Wright WH, Keitt GW, Sagen HE. 1930. Studies on infectious hairy root of nursery apple trees. J Agric Res 41:507–540.
    1. Serino G, Clerot D, Brevet J, Costantino P, Cardarelli M. 1994. rol genes of Agrobacterium rhizogenes cucumopine strain: sequence, effects and pattern of expression. Plant Mol Biol 26:415–422. doi:10.1007/BF00039550. - DOI - PubMed
    1. Chilton MD, Tepfer DA, Petit A, David C, Casse-Delbart F, Tempé J. 1982. Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells. Nature 295:432–434. doi:10.1038/295432a0. - DOI
    1. Combard A, Brevet J, Borowski D, Cam K, Tempé J. 1987. Physical map of the T-DNA region of Agrobacterium rhizogenes strain NCPPB2659. Plasmid 18:70–75. doi:10.1016/0147-619X(87)90079-5. - DOI - PubMed
    1. Mankin SL, Hill DS, Olhoft PM, Toren E, Wenck AR, Nea L, Xing L, Brown JA, Fu H, Ireland L, Jia H, Hillebrand H, Jones T, Song H-S. 2007. Disarming and sequencing of Agrobacterium rhizogenes strain K599 (NCPPB2659) plasmid pRi2659. In Vitro Cell Dev Biol Plant 43:521–535. doi:10.1007/s11627-007-9071-4. - DOI

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