doi: 10.1016/j.molbiopara.2016.07.006.
Epub 2016 Jul 26.
Comparison of a new visual isothermal nucleic acid amplification test with PCR and skin snip analysis for diagnosis of onchocerciasis in humans
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- PMID: 27473357
- DOI: 10.1016/j.molbiopara.2016.07.006
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Comparison of a new visual isothermal nucleic acid amplification test with PCR and skin snip analysis for diagnosis of onchocerciasis in humans
Mol Biochem Parasitol.
2016 Nov-Dec.
Free article
Abstract
Accurate, simple and affordable diagnostics are needed to detect Onchocerca volvulus infection in humans. A newly developed colorimetric loop-mediated isothermal amplification (LAMP) assay was compared to PCR and skin snip analysis for diagnosis of onchocerciasis. The robustness and simplicity of the assay indicates that it may be a useful field tool for surveillance in endemic countries.
Keywords: Colorimetric LAMP; Diagnosis; O-150 repeat; Onchocerciasis; Skin snips.
Copyright © 2016 The Author(s). Published by Elsevier B.V. All rights reserved.
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