Interferences with Luciferase Reporter Enzymes

Excerpt

Here we describe the molecular basis underlying interference by inhibitors of reporter enzymes for biochemical and cell-based assays. This understanding is used to create strategies to mitigate the confounding effects reporter enzyme inhibitor interference has on interpreting high throughput screening results, and to aid in evaluating chemical probes derived from reporter gene assays. As well, example compounds that act as potent inhibitors of luciferases are described which can serve as useful tools when developing luciferase-based assays.