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Comment
. 2016 Aug 1:3:160060.
doi: 10.1038/sdata.2016.60.

Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Tomas Ekeberg  1   2 , Martin Svenda  1 , M Marvin Seibert  1 , Chantal Abergel  3 , Filipe R N C Maia  1 , Virginie Seltzer  3 , Daniel P DePonte  2   4 , Andrew Aquila  4   5 , Jakob Andreasson  1 , Bianca Iwan  1   6 , Olof Jönsson  1   7 , Daniel Westphal  1 , Duško Odić  1 , Inger Andersson  1 , Anton Barty  2 , Meng Liang  2   4 , Andrew V Martin  2   8 , Lars Gumprecht  2 , Holger Fleckenstein  2 , Saša Bajt  9 , Miriam Barthelmess  9 , Nicola Coppola  2 , Jean-Michel Claverie  3 , N Duane Loh  10   11 , Christoph Bostedt  4 , John D Bozek  12 , Jacek Krzywinski  4 , Marc Messerschmidt  4 , Michael J Bogan  10 , Christina Y Hampton  10 , Raymond G Sierra  10 , Matthias Frank  13 , Robert L Shoeman  14 , Lukas Lomb  14 , Lutz Foucar  14   15 , Sascha W Epp  15   16 , Daniel Rolles  14   15   17 , Artem Rudenko  15   16   17 , Robert Hartmann  18 , Andreas Hartmann  18 , Nils Kimmel  19   20 , Peter Holl  18 , Georg Weidenspointner  19   20 , Benedikt Rudek  15   16 , Benjamin Erk  15   16 , Stephan Kassemeyer  14 , Ilme Schlichting  14   15 , Lothar Strüder  18   21 , Joachim Ullrich  15   16   22 , Carlo Schmidt  15   16 , Faton Krasniqi  14   15 , Günter Hauser  19   20 , Christian Reich  18 , Heike Soltau  18 , Sebastian Schorb  23 , Helmut Hirsemann  9 , Cornelia Wunderer  9 , Heinz Graafsma  9 , Henry Chapman  2   24 , Janos Hajdu  1   5
Affiliations
Comment

Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Tomas Ekeberg et al. Sci Data. .

Abstract

Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.

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Conflict of interest statement

The authors declare no competing financial interests.

Figures

Figure 1
Figure 1. The first 24 of the 198 diffraction patterns in this dataset.
The color scale is logarithmic and ranges from 1 to 820 photons per pixel. This is a modification of a figure previously presented in ref. .
See this image and copyright information in PMC

Comment in

Comment on

  • Three-dimensional reconstruction of the giant mimivirus particle with an x-ray free-electron laser.
    Ekeberg T, Svenda M, Abergel C, Maia FR, Seltzer V, Claverie JM, Hantke M, Jönsson O, Nettelblad C, van der Schot G, Liang M, DePonte DP, Barty A, Seibert MM, Iwan B, Andersson I, Loh ND, Martin AV, Chapman H, Bostedt C, Bozek JD, Ferguson KR, Krzywinski J, Epp SW, Rolles D, Rudenko A, Hartmann R, Kimmel N, Hajdu J. Ekeberg T, et al. Phys Rev Lett. 2015 Mar 6;114(9):098102. doi: 10.1103/PhysRevLett.114.098102. Epub 2015 Mar 2. Phys Rev Lett. 2015. PMID: 25793853

References

Data Citations

    1. Ekeberg T. 2015. Coherent X-ray Imaging Data Bank. http://dx.doi.org/10.11577/1236752 - DOI

References

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