Empirical search for factors affecting mean particle size of PLGA microspheres containing macromolecular drugs

Abstract

Background and objectives: Poly(lactic-co-glycolic acid) (PLGA) has become one of the most promising in design, development, and optimization for medical applications polymers. PLGA-based multiparticulate dosage forms are usually prepared as microspheres where the size is from 5 to 100 µm, depending on the route of administration. The main objectives of the study were to develop a predictive model of mean volumetric particle size and on its basis extract knowledge of PLGA containing proteins forming behaviour.

Methods: In the present study, a model for the prediction of mean volumetric particle size developed by an rgp package of R environment is presented. Other tools like fscaret, monmlp, fugeR, MARS, SVM, kNNreg, Cubist, randomForest and piecewise linear regression are also applied during the data mining procedure.

Results: The feature selection provided by the fscaret package reduced the original input vector from a total of 295 input variables to 10, 16 and 19. The developed models had good predictive ability, which was confirmed by a normalized root-mean-square error (NRMSE) of 6.8 to 11.1% in 10-fold cross validation training procedure. Moreover, the best models were validated using external experimental data. The superior predictiveness had a model obtained by rgp in the form of a classical equation with a normalized root-mean-squared error (NRMSE) of 6.1%.

Conclusions: A new approach is proposed for computational modelling of the mean particle size of PLGA microspheres and rules extraction from tree-based models. The feature selection leads to revealing chemical descriptor variables which are important in predicting the size of PLGA microspheres. In order to achieve better understanding in the relationships between particle size and formulation characteristics, the surface analysis method and rules extraction procedures were applied.

Keywords: Feature selection; Heuristic modelling; Molecular descriptors; Particle size; Poly-lactide-co-glycolide (PLGA) microparticles; Rules extraction.