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. 2016 Jun;19(6):670-5.

Colchicine protects rat skeletal muscle from ischemia/reperfusion injury by suppressing oxidative stress and inflammation

Liangrong Wang  1 , Yuanlu Shan  1 , Lei Chen  1 , Bi Lin  1 , Xiangqing Xiong  1 , Lina Lin  1 , Lida Jin  1
Affiliations

Colchicine protects rat skeletal muscle from ischemia/reperfusion injury by suppressing oxidative stress and inflammation

Liangrong Wang et al. Iran J Basic Med Sci. 2016 Jun.

Abstract

Objectives: Neutrophils play an important role in ischemia/reperfusion (IR) induced skeletal muscle injury. Microtubules are required for neutrophil activation in response to various stimuli. This study aimed to investigate the effects of colchicine, a microtubule-disrupting agent, on skeletal muscle IR injury in a rat hindlimb ischemia model.

Materials and methods: Twenty-one Sprague-Dawley rats were randomly allocated into three groups IR group, colchicine treated-IR (CO) group and sham operation (SM) group. Rats of both the IR and CO groups were subjected to 3 hr of ischemia by clamping the right femoral artery followed by 2 hr of reperfusion. Colchicine (1 mg/kg) was administrated intraperitoneally prior to hindlimb ischemia in the CO group. After 2 hr of reperfusion, we measured superoxide dismutase (SOD) and myeloperoxidase (MPO) activities, and malondialdehyde (MDA), tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels in the muscle samples. Plasma creatinine kinase (CK) and lactate dehydrogenase (LDH) levels were measured. We also evaluated the histological damage score and wet/dry weight (W/D) ratio.

Results: The histological damage score, W/D ratio, MPO activity, MDA, TNF-α and IL-1β levels in muscle tissues were significantly increased, SOD activity was decreased, and plasma CK and LDH levels were remarkably elevated in both the IR and CO groups compared to the SM group (P<0.05). Colchicine treatment significantly reduced muscle damage and edema, oxidative stress and levels of the inflammatory parameters in the CO group compared to the IR group (P<0.05).

Conclusion: Colchicine attenuates IR-induced skeletal muscle injury in rats.

Keywords: Colchicine; Inflammation; Muscle; Reperfusion injury; Skeletal.

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Figures

Figure 1
Figure 1
Representative images (×400) of H&E staining of skeletal muscle tissues: A, IR group; B, colchicine treatment-IR (CO) group; C, sham operation (SM) group. D: Histological damage scores of muscle tissues in three groups. * P<0.05 compared to SM; # P<0.05 compared to IR
Figure 2
Figure 2
The degrees of muscle edema in the IR group, colchicine treated-IR (CO) group and sham operation (SM) group. * P<0.05 compared to SM; # P<0.05 compared to IR
Figure 3
Figure 3
Measurement of superoxide dismutase (SOD) activity (A), malondialdehyde (DMA) level (B), myeloperoxidase (MPO) activity (C), tumor necrosis factor-α (TNF-α) level (D) and interleukin-1β (IL-1β) level (E) in skeletal muscle tissues of rats from the three groups. * P<0.05 compared to SM; # P<0.05 compared to IR
Figure 4
Figure 4
The Circulating levels of biomarkers lactate dehydrogenase (LDH, A) and creatinine kinase (CK, B) in rats from the IR group, colchicine treated-IR (CO) group and sham operation (SM) group. * P<0.05 compared to SM; # P<0.05 compared to IR
See this image and copyright information in PMC

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