Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jul;86(14):5478-82.
doi: 10.1073/pnas.86.14.5478.

Shuttle vectors for the archaebacterium Halobacterium volcanii

W L Lam  1 , W F Doolittle
Affiliations

Shuttle vectors for the archaebacterium Halobacterium volcanii

W L Lam et al. Proc Natl Acad Sci U S A. 1989 Jul.

Abstract

Progress in archaebacterial molecular biology requires tools for genetic analysis. We describe vectors that can be selected and maintained in either Halobacterium volcanii or Escherichia coli. A genetic determinant for resistance to the 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor mevinolin was isolated by "shotgun cloning" into a derivative of the endogenous H. volcanii plasmid pHV2, to form pWL2, which transforms sensitive H. volcanii to mevinolin resistance at high frequency. The resistance determinant, portions of pHV2, and an ampicillin- and tetracycline-resistance-conferring pBR322 derivative, pAT153, were ligated together to form the shuttle vectors pWL101 and pWL102. We describe conditions for the use of these vectors and provide preliminary definition of regions essential for drug resistance and for plasmid replication and maintenance.

PubMed Disclaimer

References

    1. J Bacteriol. 1987 Mar;169(3):1341-4 - PubMed
    1. Nucleic Acids Res. 1986 Sep 11;14(17):6983-7000 - PubMed
    1. Microbiol Rev. 1987 Jun;51(2):221-71 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8530-4 - PubMed
    1. J Bacteriol. 1988 Feb;170(2):653-6 - PubMed

Publication types

LinkOut - more resources