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. 2016 Dec 1;78(11):1731-1735.
doi: 10.1292/jvms.16-0217. Epub 2016 Aug 2.

Detection of kobuvirus RNA in Japanese domestic dogs

Takehisa Soma  1 , Makoto Matsubayashi, Kazumi Sasai
Affiliations

Detection of kobuvirus RNA in Japanese domestic dogs

Takehisa Soma et al. J Vet Med Sci. .

Abstract

To investigate whether kokuvirus is present in Japanese dogs, we examined the fecal samples obtained from 94 diarrheal household dogs and 50 clinically healthy kenneled dogs by RT-PCR. The gene was detected in 37.2% and 48.0% in the former and the latter, respectively, suggesting that canine kobuvirus (CaKoV) is circulating among Japanese dogs. From the result of the latter, however, CaKoV may not be a primary pathogen. Furthermore, all gene-positive dogs were purebreds aged four months or younger. This finding suggests that CaKoV endemic is confined in multi-dog environments, and the dogs have a strong age-dependent resistance to CaKoV.

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Figures

Fig. 1.
Fig. 1.
Phylogenetic tree based on the partial 3D nucleotide sequences of kobuviruses obtained by neighbor-joining method in the MEGA 6 software. Bootstrap analysis was performed with 1,000 replicates. Black dot marks represent 12 strains newly detected in this study. *Accession number: LC147654 (Ca-Nara0044-2015-JP), LC147655 (Ca-Gifu0226-2014-JP), LC147656 (Ca-Tokyo1173-2012-JP).
See this image and copyright information in PMC

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