Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jul 5:13:71-4.
doi: 10.1016/j.nmni.2016.06.017. eCollection 2016 Sep.

Real-time quantitative PCR assay with Taqman(®) probe for rapid detection of MCR-1 plasmid-mediated colistin resistance

S Chabou  1 , T Leangapichart  1 , L Okdah  1 , S Le Page  1 , L Hadjadj  1 , J-M Rolain  1
Affiliations

Real-time quantitative PCR assay with Taqman(®) probe for rapid detection of MCR-1 plasmid-mediated colistin resistance

S Chabou et al. New Microbes New Infect. .

Abstract

Here we report the development of two rapid real-time quantitative PCR assays with TaqMan(®) probes to detect the MCR-1 plasmid-mediated colistin resistance gene from bacterial isolates and faecal samples from chickens. Specificity and sensitivity of the assay were 100% on bacterial isolates including 18 colistin-resistant isolates carrying the mcr-1 gene (six Klebsiella pneumoniae and 12 Escherichia coli) with a calibration curve that was linear from 10(1) to 10(8) DNA copies. Five out of 833 faecal samples from chickens from Algeria were positive, from which three E. coli strains were isolated and confirmed to harbour the mcr-1 gene by standard PCR and sequencing.

Keywords: Antibiotic resistance surveillance; PCR detection, Taqman probe; colistin resistance; mcr-1 gene.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Real-time PCR sensitivity test to detect -encoding plasmid-mediated colistin resistance (MCR-1) encoding gene from Escherichia coli strain P10. PE1 and PE2 are two quantitative PCR assays developed.
See this image and copyright information in PMC

References

    1. Biswas S., Brunel J.M., Dubus J.C., Reynaud-Gaubert M., Rolain J.M. Colistin: an update on the antibiotic of the 21st century. Expert Rev Anti Infect Ther. 2012;10:917–934. - PubMed
    1. Rolain J.M., Olaitan A.O. Plasmid-mediated colistin resistance: the final blow to colistin? Int J Antimicrob Agents. 2016;47:4–5. - PubMed
    1. Olaitan A.O., Morand S., Rolain J.M. Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteria. Front Microbiol. 2014;5:643. - PMC - PubMed
    1. Liu Y.Y., Wang Y., Walsh T.R., Yi L.X., Zhang R., Spencer J. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16:161–168. - PubMed
    1. Olaitan A.O., Chabou S., Okdah L., Morand S., Rolain J.M. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis. 2016;16:147. - PubMed