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Review
. 2016 Sep;11(9):1573-8.
doi: 10.1038/nprot.2016.104. Epub 2016 Aug 4.

Genome editing comes of age

Jin-Soo Kim  1   2
Affiliations
Review

Genome editing comes of age

Jin-Soo Kim. Nat Protoc. 2016 Sep.

Abstract

Genome editing harnesses programmable nucleases to cut and paste genetic information in a targeted manner in living cells and organisms. Here, I review the development of programmable nucleases, including zinc finger nucleases (ZFNs), TAL (transcription-activator-like) effector nucleases (TALENs) and CRISPR (cluster of regularly interspaced palindromic repeats)-Cas9 (CRISPR-associated protein 9) RNA-guided endonucleases (RGENs). I specifically highlight the key advances that set the foundation for the rapid and widespread implementation of CRISPR-Cas9 genome editing approaches that has revolutionized the field.

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References

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