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. 2016 Sep 6;49(13):3106-3110.
doi: 10.1016/j.jbiomech.2016.07.024. Epub 2016 Jul 27.

Arthroscopic irrigation of the bovine stifle joint increases cartilage surface friction and decreases superficial zone lubricin

Erin Teeple  1 , Naga Padmini Karamchedu  2 , Katherine M Larson  3 , Ling Zhang  4 , Gary J Badger  5 , Braden C Fleming  6 , Gregory D Jay  7
Affiliations

Arthroscopic irrigation of the bovine stifle joint increases cartilage surface friction and decreases superficial zone lubricin

Erin Teeple et al. J Biomech. .

Abstract

The purpose of this study was to determine the effects of arthroscopic irrigation on cartilage superficial zone lubricin and surface friction. Arthroscopic partial meniscectomy is one of the most commonly performed orthopedic surgeries in the United States, but rates of osteoarthritis progression following this procedure are high. The effect of arthroscopic irrigation on articular surface lubrication has not been previously considered as a contributing factor in outcomes after arthroscopy. Fourteen bovine stifle joints were randomized to receive arthroscopic irrigation (n=7) or no treatment (n=7). Full-thickness osteochondral explants from these joints underwent friction testing to measure static and dynamic coefficients of friction. Following mechanical testing, samples were fixed and stained for lubricin. Percent integrated density, a measure of the amount of lubricin in the superficial zone (0-100µm depth), was determined. Static and dynamic coefficients of friction were found to be significantly greater in arthroscopy specimens compared to controls (p=0.02 and p<0.001, respectively). Percent integrated density of lubricin in the superficial zone was significantly lower in arthroscopy specimens compared to controls (p<0.001).

Keywords: Arthroscopy; Lubricin; Osteoarthritis; Osteoarthrosis; Tribosupplementation.

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Conflict of interest statement

GDJ has co-authored and was granted a United States patent (# 6743774) for the therapeutic use of lubricin in joints. GDJ receives support from a National Institutes of Health funded Phase II STTR (R42-AR057276) in these clinical translation efforts. ET has performed contract work under the above-mentioned Phase II STTR, which is separate from the contents and analysis of this manuscript. This manuscript neither materially nor financially affects Dr. Jay’s patent relating to rhPRG4 nor does it impact or include Dr. Teeple’s previously performed contract work.

Figures

Figure 1
Figure 1
Graphic showing anterior (A), middle (M), and posterior (P) cartilage harvest sites from the medial femoral condyle of a bovine stifle joint.
Figure 2
Figure 2
Mean static and dynamic coefficients of friction for control and arthroscopically irrigated cartilage explants. Static and dynamic friction was significantly higher in the arthroscopy explants compared to controls.
Figure 3
Figure 3
Mean percent integrated density of lubricin in the superficial zone (0=100um) of the medial femoral condyle for control and arthroscopically irrigated cartilage explants. Surface and superficial zone lubricin was significantly lower in arthroscopy samples compared to controls.
Figure 4
Figure 4
Representative median images of histochemical detection of PRG4 with mAb 9G3in the medial femoral condyle cartilage by region show reduced surface lubricin in arthroscopy treated samples. Scale bars indicate 20um.
See this image and copyright information in PMC

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