External RNA Controls Consortium Beta Version Update
- PMID: 27512518
- PMCID: PMC4978944
- DOI: 10.7150/jgen.16082
External RNA Controls Consortium Beta Version Update
Abstract
Spike-in RNAs are valuable controls for a variety of gene expression measurements. The External RNA Controls Consortium developed test sets that were used in a number of published reports. Here we provide an authoritative table that summarizes, updates, and corrects errors in the test version that ultimately resulted in the certified Standard Reference Material 2374. We have noted existence of anti-sense RNA controls in the material, corrected sub-pool memberships, and commented on control RNAs that displayed inconsistent behavior.
Keywords: ERCC; NIST standard reference materials.; external RNA controls; spike-in controls.
Conflict of interest statement
The authors declare no competing interests.
References
-
- Baker SC, Bauer SR, Beyer RP, Brenton JD, Bromley B, Burrill J, Causton H, Conley MP, Elespuru R, Fero M. et al. The External RNA Controls Consortium: a progress report. Nature methods. 2005;2(10):731–734. - PubMed
-
- Munro SA, Lund SP, Pine PS, Binder H, Clevert DA, Conesa A, Dopazo J, Fasold M, Hochreiter S, Hong H. et al. Assessing technical performance in differential gene expression experiments with external spike-in RNA control ratio mixtures. Nature communications. 2014;5:5125. - PubMed
LinkOut - more resources
Full Text Sources
Other Literature Sources