Transcriptomics of liver and muscle in Holstein cows genetically divergent for fertility highlight differences in nutrient partitioning and inflammation processes

Abstract

Background: The transition between pregnancy and lactation is a major physiological change for dairy cows. Complex systemic and local processes involving regulation of energy balance, galactopoiesis, utilisation of body reserves, insulin resistance, resumption of oestrous cyclicity and involution of the uterus can affect animal productivity and hence farm profitability. Here we used an established Holstein dairy cow model of fertility that displayed genetic and phenotypic divergence in calving interval. Cows had similar genetic merit for milk production traits, but either very good genetic merit for fertility traits ('Fert+'; n = 8) or very poor genetic merit for fertility traits ('Fert-'; n = 8). We used RNA sequencing to investigate gene expression profiles in both liver and muscle tissue biopsies at three distinct time-points: late pregnancy, early lactation and mid lactation (-18, 1 and 147 days relative to parturition, respectively).

Results: We found 807 and 815 unique genes to be differentially expressed in at least one time-point in liver and muscle respectively, of which 79 % and 83 % were only found in a single time-point; 40 and 41 genes were found differentially expressed at every time-point indicating possible systemic or chronic dysregulation. Functional annotation of all differentially expressed genes highlighted two physiological processes that were impacted at every time-point in the study, These were immune and inflammation, and metabolic, lipid and carbohydrate-binding.

Conclusion: These pathways have previously been identified by other researchers. We show that several specific genes which are differentially regulated, including IGF-1, might impact dairy fertility. We postulate that an increased burden of reactive oxidation species, coupled with a chronic inflammatory state, might reduce dairy cow fertility in our model.

Keywords: Dairy cow fertility; Gene expression; Lactation; Liver, muscle.

Fig. 1

Phenotypic characterization of the Fert + and Fert- cows. Solids corrected milk yield (a) and bodyweight (b) were similar in both genotypes (P > 0.9 and P > 0.3, respectively), but BCS (c) was greater (P < 0.001) in Fert + cows compared with Fert- cows. Plasma insulin (d) and IGF1 (e) were greater in Fert + cows compared with Fert- cows (P = 0.003 and P < 0.001, respectively), but plasma NEFA concentrations (f) were not different (P = 0.9)