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. 2016 Aug 10:27:31148.
doi: 10.3402/mehd.v27.31148. eCollection 2016.

A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in 6 years

Catrine Ahlen  1 , Marianne Aas  2 , Jadwiga Krusnell  3 , Ole-Jan Iversen  4
Affiliations

A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in 6 years

Catrine Ahlen et al. Microb Ecol Health Dis. .

Abstract

Background: Recurrent legionella outbreaks at one and the same location are common. We have identified a single Legionella pneumophila genotype associated with recurrent Legionella outbreaks over 6 years.

Methods: Field emergency surveys following Legionella outbreaks were performed on a vessel in 2008, 2009 and 2013. Water samples from both the distribution and technical parts of the potable water system were analyzed with respect to L. pneumophila [Real-Time PCR, cultivation, serotyping and genotyping (PFGE)] and free-living amoebae, (FLA).

Results: Legionella pneumophila serogroup 1 was present in the ship's potable water system during every outbreak. Genotyping of the 2008 survey material showed two separate PFGE genotypes while those in 2009 and 2013 demonstrated the presence of only one of the two genotypes. FLA with intracellular L. pneumophila of the same genotype were also detected. Analyses of the freshwater system on a ship following three separate Legionella outbreaks, for L. pneumophila and FLAs, revealed a single L. pneumophila genotype and FLA (Hartmanella).

Conclusions: It is reasonable to assume that the L. pneumophila genotype detected in the freshwater system was the causal agent in the outbreaks onboard. Persistence of an apparently low-pathogenic L. pneumophila genotype and FLA in a potable water system represent a potential risk for recurrent outbreaks.

Keywords: Free-living amoeba; Genotypes; Legionella pneumophila; Pathogenicity; Prevention and control; Ships; Virulence; infection outbreaks.

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Figures

Fig. 1
Fig. 1
The results from genotyping of the first 18 Legionella pneumophila isolates analysed from the 2008 survey. Two separate genotypes of L. pneumophila (Lp-gta and Lp-gtb) identified in the potable water distribution system. Lane 1, 11 and 21 are Lambda Stds. Lanes 2–5, 7, 8, 10, 13, 15, 19–20 are Lp-gta. Lanes 6, 9, 12, 14, 16–18 are Lp-gtb.
Fig. 2
Fig. 2
The results from genotyping of the five Legionella pneumophila isolates analysed from the 2009 survey. Only one genotype of L. pneumophila (Lp-gta) was seen in the potable water distribution system by the time for outbreak. Lanes 1 and 9 are Lambda Stds. Lanes 2–6 are isolates from the 2009 survey and show Lp-gta. Lanes 7 and 8 are Lp-gta isolates from the 2008 survey.
Fig. 3
Fig. 3
The results from genotyping of random Legionella pneumophila isolates analysed from the 2013 survey. Only one genotype of L. pneumophila (Lp-gta) was seen in the potable water distribution system by the time for the survey. Lanes 1 and 11 are Lambda Stds. Lanes 2–10 are random isolates from the 2013 survey, all isolates Lp-gta.
See this image and copyright information in PMC

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