[Purification, quaternary structure and regulatory properties of phosphorylase kinase from pigeon skeletal muscle]
- PMID: 2752064
[Purification, quaternary structure and regulatory properties of phosphorylase kinase from pigeon skeletal muscle]
Abstract
Using DEAE-Toyopearl column chromatography, a preparation of pigeon skeletal muscle phosphorylase kinase was obtained in a state approaching homogeneity. The molecular mass of the native enzyme (1320 kDa) and the subunit formula (alpha beta gamma delta)4 are similar to those of rabbit and chicken counterparts. Both red and white pigeon skeletal muscle isozymes contain the alpha'-subunit instead of alpha. Gradient SDS-PAGE electrophoresis revealed small but well-reproducible differences in the molecular masses of rabbit, chicken and pigeon muscle beta- and gamma-subunits. The activity ratio at pH 6.8/8.2 is 0.06-0.15 for different preparations of phosphorylase kinase b. The activity of pigeon muscle phosphorylase kinase b is Ca2+-dependent. The [Ca2+]0.5 value at pH 7.0 is 20 microM, which exceeds that for the chicken muscle enzyme by two orders of magnitude. In the presence of Ca2+, pigeon phosphorylase kinase b is activated 4-fold by saturating concentrations of calmodulin and troponin C. Pigeon muscle phosphorylase b is activated 3-5-fold during autophosphorylation or phosphorylation by the catalytic subunit of cAMP-dependent protein kinase.
Similar articles
-
[Molecular mechanisms of the regulation of phosphorylase kinase from skeletal muscles of mammals and birds].Biokhimiia. 1989 May;54(5):730-3. Biokhimiia. 1989. PMID: 2758078 Russian.
-
Phosphorylase kinase from chicken skeletal muscle. Quaternary structure, regulatory properties and partial proteolysis.Eur J Biochem. 1986 Jul 1;158(1):99-106. doi: 10.1111/j.1432-1033.1986.tb09726.x. Eur J Biochem. 1986. PMID: 3089780
-
[Regulatory properties of phosphorylase from chicken skeletal muscle].Biokhimiia. 1985 Oct;50(10):1646-52. Biokhimiia. 1985. PMID: 4074775 Russian.
-
[Structure and regulation of the activity of muscle phosphorylase kinase].Biokhimiia. 1993 Nov;58(11):1677-84. Biokhimiia. 1993. PMID: 8268309 Review. Russian.
-
[Quaternary structure of NAD-kinase from rabbit skeletal muscles].Ukr Biokhim Zh. 1977 Jul-Aug;49(4):124-9. Ukr Biokhim Zh. 1977. PMID: 197673 Review. Russian.
Cited by
-
Self-association of phosphorylase kinase from rabbit skeletal muscles under conditions close to those of an intracellular environment.Dokl Biochem Biophys. 2007 Jul-Aug;415:183-5. doi: 10.1134/s1607672907040059. Dokl Biochem Biophys. 2007. PMID: 17933330 No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Miscellaneous