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. 2016 Dec;17(4):585-592.
doi: 10.1007/s10561-016-9569-2. Epub 2016 Aug 13.

Disinfection of human skin allografts in tissue banking: a systematic review report

C Johnston  1 , J Callum  2 , J Mohr  3 , A Duong  4 , A Garibaldi  4 , N Simunovic  4 , O R Ayeni  5   6 ; Bioburden Steering Committee and Skin Working group
Collaborators, Affiliations

Disinfection of human skin allografts in tissue banking: a systematic review report

C Johnston et al. Cell Tissue Bank. 2016 Dec.

Abstract

The use of skin allografts to temporarily replace lost or damaged skin is practiced worldwide. Naturally occurring contamination can be present on skin or can be introduced at recovery or during processing. This contamination can pose a threat to allograft recipients. Bacterial culture and disinfection of allografts are mandated, but the specific practices and methodologies are not dictated by standards. A systematic review of literature from three databases found 12 research articles that evaluated bioburden reduction processes of skin grafts. The use of broad spectrum antibiotics and antifungal agents was the most frequently identified disinfection method reported demonstrating reductions in contamination rates. It was determined that the greatest reduction in the skin allograft contamination rates utilized 0.1 % peracetic acid or 25 kGy of gamma irradiation at lower temperatures.

Keywords: Bioburden; Skin allografts; Skin decontamination; Tissue banking; Tissue donation.

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Conflict of interest statement

Compliance with ethical standards Conflicts of interest No conflicts-of-interest were identified on disclosure forms completed by members of the Committee.

Figures

Fig. 1
Fig. 1
Screening process flow diagram
See this image and copyright information in PMC

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