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. 2016 Sep:6:113-117.
doi: 10.1016/j.jgar.2016.04.008. Epub 2016 May 30.

Whole-genome sequencing of multidrug-resistant Mycobacterium tuberculosis isolates from Myanmar

Htin Lin Aung  1 , Thanda Tun  2 , Danesh Moradigaravand  3 , Claudio U Köser  3 , Wint Wint Nyunt  4 , Si Thu Aung  4 , Thandar Lwin  4 , Kyi Kyi Thinn  5 , John A Crump  6 , Julian Parkhill  7 , Sharon J Peacock  8 , Gregory M Cook  9 , Philip C Hill  6
Affiliations

Whole-genome sequencing of multidrug-resistant Mycobacterium tuberculosis isolates from Myanmar

Htin Lin Aung et al. J Glob Antimicrob Resist. 2016 Sep.

Abstract

Drug-resistant tuberculosis (TB) is a major health threat in Myanmar. An initial study was conducted to explore the potential utility of whole-genome sequencing (WGS) for the diagnosis and management of drug-resistant TB in Myanmar. Fourteen multidrug-resistant Mycobacterium tuberculosis isolates were sequenced. Known resistance genes for a total of nine antibiotics commonly used in the treatment of drug-susceptible and multidrug-resistant TB (MDR-TB) in Myanmar were interrogated through WGS. All 14 isolates were MDR-TB, consistent with the results of phenotypic drug susceptibility testing (DST), and the Beijing lineage predominated. Based on the results of WGS, 9 of the 14 isolates were potentially resistant to at least one of the drugs used in the standard MDR-TB regimen but for which phenotypic DST is not conducted in Myanmar. This study highlights a need for the introduction of second-line DST as part of routine TB diagnosis in Myanmar as well as new classes of TB drugs to construct effective regimens.

Keywords: Drug-resistant TB; Myanmar; Whole-genome sequencing.

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