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. 2016 Sep;22(9):1635-9.
doi: 10.3201/eid2209.151969.

Large-Scale Survey for Tickborne Bacteria, Khammouan Province, Laos

Andrew J Taylor, Khamsing Vongphayloth, Malavanh Vongsouvath, Marc Grandadam, Paul T Brey, Paul N Newton, Ian W Sutherland, Sabine Dittrich

Large-Scale Survey for Tickborne Bacteria, Khammouan Province, Laos

Andrew J Taylor et al. Emerg Infect Dis. 2016 Sep.

Erratum in

  • Correction: Vol. 22, No. 9.
    [No authors listed] [No authors listed] Emerg Infect Dis. 2016 Nov;22(11):2028. doi: 10.3201/eid2211.C12211. Emerg Infect Dis. 2016. PMID: 31265532 Free PMC article.

Abstract

We screened 768 tick pools containing 6,962 ticks from Khammouan Province, Laos, by using quantitative real-time PCR and identified Rickettsia spp., Ehrlichia spp., and Borrelia spp. Sequencing of Rickettsia spp.-positive and Borrelia spp.-positive pools provided evidence for distinct genotypes. Our results identified bacteria with human disease potential in ticks in Laos.

Keywords: Amblyomma; Anaplasma; Borrelia; Coxiella; Dermacentor; Ehrlichia; Haemaphysalis; Laos; Rickettsia; Southeast Asia; bacteria; survey; tickborne bacteria; ticks; vector-borne infections.

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Figures

Figure 1
Figure 1
Phylogenetic analysis of Rickettsia spp. in ticks, Khammouan Province, Laos. The tree was constructed by using partial nucleotide sequences (350 bp) of the 17-kDa gene, the Kimura 2-parameter model, and the neighbor-joining method. Analyses were supported by bootstrap analysis with 1,000 replications. Numbers along branches are bootstrap values. GenBank accession numbers are shown for reference sequences. Sample numbers for each tick are shown in parentheses. Scale bar indicates nucleotide substitutions per site.
Figure 2
Figure 2
Phylogenetic analysis of Borrelia spp. in ticks, Khammouan Province, Laos. The tree was constructed by using partial nucleotide sequences (299–323 bp) of the flaB gene, the Kimura 2-parameter model, and the neighbor-joining method. Analyses were supported by bootstrap analysis with 1,000 replications. Numbers along branches are bootstrap values. GenBank accession numbers are shown for reference sequences. Sample numbers for each tick are shown in parentheses. Scale bar indicates nucleotide substitutions per site.
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