Mass spectrometry based data of the blister fluid proteome of paediatric burn patients

Abstract

The data presented here are associated with the article "The blister fluid proteome of paediatric burns" (Zang et al., 2016) [1]. Burn injury is a highly traumatic event for children. The degree of burn severity (superficial-, deep-, or full-thickness injury) often dictates the extent of later scar formation which may require long term surgical operation or skin grafting. The data were obtained by fractionating paediatric burn blister fluid samples, which were pooled according to burn depth and then analysed using data dependent acquisition LC-MS/MS. The data includes a table of all proteins identified, in which burn depth category they were found, the percentage sequence coverage for each protein and the number of high confidence peptide identifications for each protein. Further Gene Ontology enrichment analysis shows the significantly over-represented biological processes, molecular functions, and cellular components of the burn blister fluid proteome. In addition, tables include the proteins associated with the biological processes of "wound healing" and "response to stress" as examples of highly relevant processes that occur in burn wounds.

Fig. 1

The mass spectra of an example sample. (A) Total ion chromatogram (blue) shows the complexity of ion information acquired from a single blister fluid sample. The dependent scan chromatogram (pink) shows the number of MS/MS ions detected. The total protein sequence coverage obtained and the corresponding MS2 spectra, with good y-ion series, of the underlined peptide are exemplified for burn relevant proteins, haemoglobin subunit alpha (B) and alpha-2 macroglobulin (C), respectively. Sequence coverage indicated by ≥95% confident peptides (green), ≥75% confident peptides (yellow), and ≥50% confident peptides (red). Grey amino acids were not detected.

Fig. 2

Gene ontology enrichment analysis for the entire protein inventory of burn blister fluid. The number of proteins involved in the annotation is in proportion to the size of nodes. The colour of the node represents the (corrected) p-value with a darker colour indicative of greater significance of over-representation for that GO term. Uncoloured nodes are the parents of over-represented downstream categories without over-representation themselves. (A) The network of biological process. (B) The network of cellular component. (C) The network of molecular function.