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Comparative Study
. 1989 Aug;171(8):4511-3.
doi: 10.1128/jb.171.8.4511-4513.1989.

Pulsed-field gel electrophoresis determination of the genome size of obligate intracellular bacteria belonging to the genera Chlamydia, Rickettsiella, and Porochlamydia

R Frutos  1 , M Pages, M Bellis, G Roizes, M Bergoin
Affiliations
Comparative Study

Pulsed-field gel electrophoresis determination of the genome size of obligate intracellular bacteria belonging to the genera Chlamydia, Rickettsiella, and Porochlamydia

R Frutos et al. J Bacteriol. 1989 Aug.

Abstract

The chromosome length of obligate intracellular procaryotes was determined by pulsed-field gel electrophoresis of intact or NotI- and SfiI-restricted genomes. Sizes averaged 2,100, 1,720, 1,550, 2,650, and 1,450 kilobases for Rickettsiella grylli, Rickettsiella melolonthae, Porochlamydia buthi, Porochlamydia chironomi, and Chlamydia psittaci and Chlamydia trachomatis, respectively. An SfiI restriction map of the R. melolonthae genome was derived.

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