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. 2019:1576:205-213.
doi: 10.1007/7651_2016_4.

Oncogenic Transformation of Human-Derived Gastric Organoids

Nina Bertaux-Skeirik  1 , Jomaris Centeno  2 , Jian Gao  3 , Joel Gabre  4 , Yana Zavros  5
Affiliations

Oncogenic Transformation of Human-Derived Gastric Organoids

Nina Bertaux-Skeirik et al. Methods Mol Biol. 2019.

Abstract

The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.

Keywords: CRISPR/Cas-9; Human fundic gastric organoids; Nucleofection; Organoid orthotopic transplantation.

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