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. 2016 Jan;23(1):15-21.

Effect of C-peptide Alone or in Combination with Nicotinamide on Insulin Levels from Pancreatic Islets in Mouse

Akram Ahangarpour  1 , Fatemeh Ramezani Ali Akbari  2 , Hadi Fathi Moghadam  3
Affiliations

Effect of C-peptide Alone or in Combination with Nicotinamide on Insulin Levels from Pancreatic Islets in Mouse

Akram Ahangarpour et al. Malays J Med Sci. 2016 Jan.

Abstract

Background: Both c-peptide and nicotinamide are known to increase blood insulin in diabetes. In the present study, we examined the effect of c-peptide alone or in combination with nicotinamide on insulin levels in pancreatic islets in mice.

Methods: This study was conducted with 60 adult male Naval Medical Research Institute (NMARI) mice weighing 25 to 30 g. Pancreatic islets from normal mice were isolated by the collagenase digestion method. Mice were divided into ten groups of six (n = 6): control, glyburide (1 and 10 μM), C-peptide (50 and 100 nM), nicotinamide (10, 25, and 100 mM), nicotinamide + C-peptide (100 mM and 100 nM), and buffer in different glucose concentrations (2.8, 5.6, and 16.7 mM). Insulin secretion was measured using insulin radioimmunoassay method.

Results: Insulin secretion significantly increased at 16.7 mM glucose concentration compared with 2.8 and 5.6 mM glucose concentrations. Incubation of islets at 2.8 and 5.6 mM glucose concentrations and nicotinamide + C-peptide, nicotinamide 25 and 100 mM, and C-peptide 100 nM significantly increased insulin secretion compared with the control group. In addition, incubation of islets at 16.7 mM glucose with nicotinamide + C-peptide significantly increased insulin secretion. Glyburide at 10 μM concentration was more effective than nicotinamide at 10 and 100 mM, C-peptide 50 and 100 nM in the presence of 16.7 mM glucose concentration. However, the combination of nicotinamide + C-peptide was more effective than glyburide at a concentration of 10 μM in the presence of a 16.7 mM glucose concentration.

Conclusions: This paper suggests that c-peptide, nicotinamide, and the combination of c-peptide and nicotinamide in-creases insulin secretion from pancreatic islets.

Keywords: C-peptide; glyburide; insulin; islets; mice; nicotinamide.

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Figures

Figure 1
Figure 1
The effects of saline and buffer with different concentrations of glucose on insulin secretion from pancreatic islets (mean ± SD). n = 6, **P < 0.01, ***P < 0.001 when compared with buffer group, ++ P < 0.01, +++ P < 0.001 when compared with 2.8 group.
Figure 2
Figure 2
The effects of C-peptide (cp) at 50 and 100 nM, nicotinamide (na) at 10, 25, and 100 mM, nicotinamide + C-peptide [c(100nM) + n(100mM)] and glyburide (gly) at 1 and 10 μM with 2.8 glucose concentration on insulin secretion. N = 6, *P < 0.05, **P < 0.01, ***P < 0.001 whereas compared with saline group, F ratio 12.27.
Figure 3
Figure 3
The effects of C-peptide (cp) at 50 and 100 nM, nicotinamide (na) at 10, 25, and 100 mM, nicotinamide + C-peptide [c(100nM)+n(100mM)] and glyburide (gly) at 1 and 10 μM with 5.6 glucose concentration on insulin secretion. N = 6, **P < 0.01, ***P < 0.001 when compared with saline group, F ratio 7.80.
Figure 4
Figure 4
The effects of C-peptide (cp) at 50 and 100 nM, nicotinamide (na) at 10, 25, and 100 mM, Nicotinamide + C-peptide [c(100nM) + n(100mM)] and glyburide (gly) at 1 and 10 μM with 16.7 glucose concentration on insulin secretion., n = 6, ***P < 0.001 when compared with saline group, F ratio 29.79.
Figure 5
Figure 5
Staining isolated islets by Dithizone in the culture medium.
See this image and copyright information in PMC

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