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. 2016 Aug 5;5(1):1274.
doi: 10.1186/s40064-016-2919-5. eCollection 2016.

The role of growth factors on hepatic damage in rats with obstructive jaundice

Ozgur Turk  1 , Bartu Badak  2 , Ersin Ates  2 , Emine Dundar  3 , Emine Sutken  4
Affiliations

The role of growth factors on hepatic damage in rats with obstructive jaundice

Ozgur Turk et al. Springerplus. .

Abstract

In this study, we investigated the affect and the role of growth factors on liver damage. 110 Sprague-Dawley rats were divided into 11 groups: a sham group, a control group, HGF, EGF, IGF, TGF groups of irreversible jaundiced rats and a control group and HGF, EGF, IGF, and TGF groups of reversible jaundiced rats (n = 10). In the irreversible jaundiced groups, the common bile duct was explorated, double ligated, and cut. 150 μg/kg/day HGF, 5 μg/kg/day EGF, 5 μg/kg/day IGF, and 5 μg/kg/day TGF β-1 were injected intraperitoneally after the seventh post-operative day. In the reversible jaundiced group, the common bile duct was ligated and the ligation was resolved on the seventh post-operative day. For 5 days, growth factors were injected at the same dose. Ductal proliferation scores significantly decreased after growth factor administration in the EGF-A and TGF-A groups. Furthermore, ductal proliferation was decreased in the TGF-B group. As a result of this study, HGF was effective in the irreversible jaundiced groups and ineffective in the reversible jaundice groups. EGF was effective in the reversible jaundiced groups and ineffective in the irreversible jaundiced groups. In both the irreversible jaundiced and reversible jaundiced groups, IGF was ineffective, although TGF β-1 was effective. We believe that these results arise from the positive effects of effective doses of growth factor on liver damage.

Keywords: Growth factors; Hepatic injury; Obstructive jaundice.

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Figures

Fig. 1
Fig. 1
Tissue MDA levels in all groups. EGF A groups MDA level was significantly high than EGF B group. IGF A group’s MDA level was significantly high than IGF B group. (p < 0.05)
Fig. 2
Fig. 2
Liver tissue antioxidant assay levels. In all groups there was no significantly difference in antioxidant assay levels
Fig. 3
Fig. 3
Histopathological evaluation of the liver tissues of rats (Hematoxylin and Eosin, ×100). a In control A group ductal proliferation score was significantly increased. Central necrosis, piecemeal necrosis and focal necrosis areas defined. b In EGF-A group. Fibrosis expansion areas seen in portal area. c In TGF-A group. Ductal proliferation and portal area fibsosis. d In TGF-B group. Ductal proliferation and portal area expansion
Fig. 4
Fig. 4
Histopathological evaluation of the liver tissues of rats (Masson’s Trichrome, ×100) a In control A group ductal proliferation score was significantly increased. Central necrosis, piecemeal necrosis and focal necrosis areas defined. b In EGF-A group. Fibrosis expansion areas seen in portal area. c In TGF-A group. Ductal proliferation and portal area fibsosis. d In TGF-B group. Ductal proliferation and portal area expansion
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