Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Aug 19:17:352.
doi: 10.1186/s12891-016-1220-7.

The effects of dopamine receptor 2 expression on B cells on bone metabolism and TNF-α levels in rheumatoid arthritis

Lei Wei  1 , Ying Sun  1 , Xiu-Fang Kong  1 , Chi Zhang  2 , Tao Yue  3 , Qi Zhu  3 , Dong-Yi He  3 , Lin-Di Jiang  4   5
Affiliations

The effects of dopamine receptor 2 expression on B cells on bone metabolism and TNF-α levels in rheumatoid arthritis

Lei Wei et al. BMC Musculoskelet Disord. .

Abstract

Background: Dopamine receptor 2 (DR2) expressions on B cells from Rheumatoid arthritis (RA) patients has been found to be negatively correlated with disease activity and can potentially predict the response to treatment. This study aimed to investigate the role of B cell DR2 expression on bone remodeling in RA.

Methods: Patients with RA (n = 14) or osteoarthritis (OA; n = 12), and healthy controls (n = 12) were recruited for this study. Dopamine receptor (DR) 2 expression was assessed using flow cytometry. Pro-inflammatory cytokines, including interleuin(IL)-1β, IL-6, IL-17, and tumor necrosis factor(TNF)-α, and bone turnovers, including osteocalcin (OC),serum procollagen type I N propeptide (PINP), C-terminal telopeptide of type I collagen (β-CTX), collagen type I cross-linked telopeptide (ICTP), as well as matrix metalloproteinase-3 (MMP-3) and osteoprotegerin (OPG) were measured by electrochemiluminescence, chemiluminescence, or enzyme-linked immunosorbent assay. DR2 expression on synovial B cells from 4 RA patients and 3 OA patients was detected by immunofluorescence.

Results: There were more DR2(+)CD19(+) B cells in synovial tissues from RA patients than in those from OA patients. The frequency of peripheral B cells that expressed DR2 was positively correlated with plasma TNF-α level. Levels of ICTP and MMP-3 were significantly higher, and OPG were lower in RA patients compared to those in the OA group and healthy controls (all P < 0.05).

Conclusion: The frequency of B cells that expressed DR2 showed a correlation with levels of the pro-inflammatory cytokine TNF-α. DR2(+)CD19(+) B cells in synovial tissues might have a role in bone metabolism and TNF-α production.

Keywords: B cells; Bone metabolism; Dopamine receptor; Rheumatoid arthritis.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Representative FACS plots of DR2-expressing cells in patients with RA, OA and healthy controls
Fig. 2
Fig. 2
Spearman correlation analysis of DR2 expression and TNF-α level in RA patients
Fig. 3
Fig. 3
In-situ immunofluorescence stainings of B cell DR2 expressions in the synovial tissues. a and b Representative images of DR2 expression on B cells from RA (N = 4) and OA (N = 3) patients are shown. Sections were counterstained with DAPI (blue) to allow visualization on nuclei; magnification, 400×. DR2-positive cells are marked by an arrowhead
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Takayanagi H. Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat Rev Immunol. 2007;7:292–304. doi: 10.1038/nri2062. - DOI - PubMed
    1. Jones SM, Bhalla AK. Osteoporosis in rheumatoid arthritis. Clin Exp Rheumatol. 1993;11:557–62. - PubMed
    1. Manilay JO, Zouali M. Tight relationships between B lymphocytes and the skeletal system. Trends Mol Med. 2014;20:405–12. doi: 10.1016/j.molmed.2014.03.003. - DOI - PubMed
    1. Mix E, Goertsches R, Zettl UK. Immunology and neurology. J Neurol. 2007;254:II2–7. doi: 10.1007/s00415-007-2002-9. - DOI - PubMed
    1. Pacheco R, Contreras F, Zouali M. The dopaminergic system in autoimmune diseases. Front Immunol. 2014;5:117. doi: 10.3389/fimmu.2014.00117. - DOI - PMC - PubMed

Publication types