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. 2016 Oct 21;60(11):6899-6902.
doi: 10.1128/AAC.01365-16. Print 2016 Nov.

Emergence of NDM-5- and MCR-1-Producing Escherichia coli Clones ST648 and ST156 from a Single Muscovy Duck (Cairina moschata)

Run-Shi Yang  1   2 , Youjun Feng  3 , Xiao-Yue Lv  1   2 , Jia-Hong Duan  1   2 , Jing Chen  1   2 , Liang-Xing Fang  1   2 , Jing Xia  1   2 , Xiao-Ping Liao  1   2 , Jian Sun  4   2 , Ya-Hong Liu  4   2
Affiliations

Emergence of NDM-5- and MCR-1-Producing Escherichia coli Clones ST648 and ST156 from a Single Muscovy Duck (Cairina moschata)

Run-Shi Yang et al. Antimicrob Agents Chemother. .

Abstract

Two Escherichia coli clones (sequence type 648 [ST648] and ST156) that coproduce NDM-5 and MCR-1 were detected from a single fowl in China. The blaNDM-5 gene was found on the two indistinguishable IncX3 plasmids from the two different E. coli isolates, whereas the mcr-1 gene was located on IncHI2 and IncI2 plasmids, respectively, suggesting that blaNDM-5 and mcr-1 have spread in avian intestinal flora. Also, the two strains harbor blaTEM-1, blaCTX-M-55, fosA3, and aac(6')-Ib The multiresistant E. coli strains (especially the epidemic clone ST648) might raise a potential threat to human health via food chain transmission.

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Figures

FIG 1
FIG 1
(a) Clonal relationship of the NDM-5- and MCR-1-coproducing clones (E. coli NDM131 and E. coli NDM132) by PFGE. Lane 1, E. coli NDM131; lane 2, E. coli NDM132; lane M, XbaI-digested genomic DNA of reference Salmonella enterica serotype Braenderup, strain H9812. Numbers on the right are molecular masses in kilodaltons. (b) Schematic representations of the genetic organization surrounding blaNDM-5 on E. coli NDM131 and E. coli NDM132. (c) Schematic representations of the genetic organization surrounding mcr-1 on E. coli NDM131 and E. coli NDM132. The arrows indicate open reading frames, and resistance genes, insertion sequences, and accessory genes are indicated by red, green, and gray arrows, respectively. The numbers in parentheses are accession numbers.
See this image and copyright information in PMC

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