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. 2016 Aug 23;12(1):174.
doi: 10.1186/s12917-016-0805-6.

Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis

Alicja Majewska  1 , Małgorzata Gajewska  2 , Kourou Dembele  3 , Henryk Maciejewski  4 , Adam Prostek  2 , Michał Jank  5
Affiliations

Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis

Alicja Majewska et al. BMC Vet Res. .

Abstract

Background: Canine atopic dermatitis (cAD) is a common chronic and pruritic skin disease in dogs. The development of cAD involves complex interactions between environmental antigens, genetic predisposition and a number of disparate cell types. The aim of the present study was to perform comprehensive analyses of peripheral blood of AD dogs in relation to healthy subjects in order to determine the changes which would be characteristic for cAD.

Results: The number of cells in specific subpopulations of lymphocytes was analyzed by flow cytometry, concentration of chosen pro- and anti-inflammatory cytokines (IL-4, IL-10, IL-13, TNF-α, TGF-β1) was determined by ELISA; and microarray analysis was performed on RNA samples isolated from peripheral blood nuclear cells of AD and healthy dogs. The number of Th cells (CD3(+)CD4(+)) in AD and healthy dogs was similar, whereas the percentage of Tc (CD3(+)CD8(+)) and Treg (CD4(+)CD25(+) Foxp3(+)) cells increased significantly in AD dogs. Increased concentrations of IL-13 and TNF-α, and decreased levels of IL-10 and TGF-β1 was observed in AD dogs. The level of IL-4 was similar in both groups of animals. Results of the microarray experiment revealed differentially expressed genes involved in transcriptional regulation (e.g., transcription factors: SMAD2, RORA) or signal transduction pathways (e.g., VEGF, SHB21, PROC) taking part in T lymphocytes lineages differentiation and cytokines synthesis.

Conclusions: Results obtained indicate that CD8(+) T cells, beside CD4(+) T lymphocytes, contribute to the development of the allergic response. Increased IL-13 concentration in AD dogs suggests that this cytokine may play more important role than IL-4 in mediating changes induced by allergic inflammation. Furthermore, observed increase in Treg cells in parallel with high concentrations of TNF-α and low levels of IL-10 and TGF-β1 in the peripheral blood of AD dogs point at the functional insufficiency of Treg cells in patients with AD.

Keywords: Canine atopic dermatitis; Cytokines; Lymphocytes; Microarray; Peripheral blood.

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Figures

Fig. 1
Fig. 1
Concentration of cytokines in plasma of dogs with atopic dermatitis and healthy dogs. Symbol * represents the level of significance: P ≤ 0.05
Fig. 2
Fig. 2
Expression of PIAS1, RORA, SH2B1 genes in peripheral blood nuclear cells of AD and healthy dogs analyzed using microarray and real-time PCR
Fig. 3
Fig. 3
Interactions between cytokines and selected genes differentially expressed in AD and healthy dogs. Detailed network of interactions generated using Pathway Studio analysis between genes showing differences in expression in peripheral blood nuclear cells of AD and healthy dogs (green highlights) and cytokines investigated in this study
See this image and copyright information in PMC

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References

    1. Olivry T, DeBoer DJ, Griffin CE, Halliwell RE, Hill PB, Hillier A, et al. The ACVD task force on canine atopic dermatitis: forewords and lexicon. Vet Immunol Immunopathol. 2001;81(3-4):143–6. doi: 10.1016/S0165-2427(01)00343-9. - DOI - PubMed
    1. Marsella R, Olivry T, Carlotti DN, International Task Force on Canine Atopic Dermatitis Current evidence of skin barrier dysfunction in human and canine atopic dermatitis. Vet Dermatol. 2011;22(3):239–48. doi: 10.1111/j.1365-3164.2011.00967.x. - DOI - PubMed
    1. Bieber T. Atopic dermatitis. N Engl J Med. 2008;358:1483–94. doi: 10.1056/NEJMra074081. - DOI - PubMed
    1. Nuttall TJ, Knight PA, McAleese SM, Lamb JR, Hill PB. Expression of Th1, Th2 and immunosuppressive cytokine gene transcripts in canine atopic dermatitis. Clin Exp Allergy. 2002;32(5):789–95. doi: 10.1046/j.1365-2222.2002.01356.x. - DOI - PubMed
    1. Schlotter YM, Rutten VP, Riemers FM, Knol EF, Willemse T. Lesional skin in atopic dogs shows a mixed Type-1 and Type-2 immune responsiveness. Vet Immunol Immunopathol. 2011;143(1-2):20–6. doi: 10.1016/j.vetimm.2011.05.025. - DOI - PubMed