Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Aug 25;11(8):e0161775.
doi: 10.1371/journal.pone.0161775. eCollection 2016.

Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma

Chunhui Yang  1 , Guohui Bian  1 , Hong Yang  1 , Xinmin Zhang  2 , Limin Chen  1 , Jingxing Wang  1
Affiliations

Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma

Chunhui Yang et al. PLoS One. .

Abstract

High hydrostatic pressure has been used to inactivate pathogens in foods for decades. There is a great potential to adapt this technology to inactivate pathogens in plasma and derivatives. To better evaluate the potential of this method, pathogen inoculated plasma samples were pressurized under different pressure application modes and temperatures. The inactivation efficacy of pathogens and activities of plasma proteins were monitored after treatment. The CFUs of E.coli was examined as the indicator of the inactivation efficiency. The factor V and VIII were chosen as the indicator of the plasma function. Preliminary experiments identified optimized treatment conditions: 200-250MPa, with 5×1 minute multi-pulsed high pressure at near 0°C (ice-water bath). Under this conditions, the inactivation efficacy of EMCV was >8.5log. The CFUs of E. coli were reduced by 7.5log, B. cereus were 8log. However, PPV and S. aureus cannot be inactivated efficiently. The activities of factor II, VII, IX, X, XI, XII, fibrinogen, IgG, IgM stayed over 95% compared to untreated. Factor V and VIII activity was maintained at 46-63% and 77-82%, respectively.

PubMed Disclaimer

Conflict of interest statement

I have read the journal’s policy and the authors of this manuscript have the following competing interests: ZX is employed by Zhengzhou Feilong medic devices Co,. Ltd. This does not alter our adherence to PLOS ONE policies on sharing data and materials.

Figures

Fig 1
Fig 1. Inactivation of E.coli under mpHHP (5 cycles of 1 min) and spHHP (1 cycle of 5 min) application modes with escalated pressure, n = 3.
Fig 2
Fig 2. Change of activities of coagulation factor V and VIII under mpHHP treatments (5 cycles of 1 min) with escalated pressure (≥ 300MPa), n = 3.
Fig 3
Fig 3. Inactivation of E.coli under two mpHHP modes (200/250 Mpa, 5 cycles of 1min) at low temperature (near 0°C) and room temperature, n = 3.
Fig 4
Fig 4. Activities of factor V and VIII after mpHHP treatment (200/250 Mpa, 5 cycles of 1min) at near 0°C temperature, n = 3.
Fig 5
Fig 5. Kinetics of virus inactivation by optimized HHP treatment (200/250 Mpa, 5 cycles of 1min, at near 0°C temperature), n = 3.
Fig 6
Fig 6. Kinetics of bacterium inactivation by optimized HHP treatment (200/250 Mpa, 5 cycles of 1min, at near 0°C temperature), n = 3.
Fig 7
Fig 7. Activities of coagulation factors after optimized HHP treatment (200/250 Mpa, 5 cycles of 1min, at near 0°C temperature), n = 3.
See this image and copyright information in PMC

References

    1. Epstein JS. Alternative strategies in assuring blood safety: An overview. Biologicals: journal of the International Association of Biological Standardization. 2010;38(1):31–5. 10.1016/j.biologicals.2009.10.009 . - DOI - PubMed
    1. Dodd RY. Emerging pathogens in transfusion medicine. Clinics in laboratory medicine. 2010;30(2):499–509. 10.1016/j.cll.2010.02.007 . - DOI - PMC - PubMed
    1. Salunkhe V, van der Meer PF, de Korte D, Seghatchian J, Gutierrez L. Development of blood transfusion product pathogen reduction treatments: a review of methods, current applications and demands. Transfusion and apheresis science: official journal of the World Apheresis Association: official journal of the European Society for Haemapheresis. 2015;52(1):19–34. 10.1016/j.transci.2014.12.016 . - DOI - PubMed
    1. Marietou A, Bartlett DH. Effects of high hydrostatic pressure on coastal bacterial community abundance and diversity. Applied and environmental microbiology. 2014;80(19):5992–6003. 10.1128/AEM.02109-14 - DOI - PMC - PubMed
    1. Barshtein G, Bergelson L, Dagan A, Gratton E, Yedgar S. Membrane lipid order of human red blood cells is altered by physiological levels of hydrostatic pressure. The American journal of physiology. 1997;272(1 Pt 2):H538–43. . - PubMed