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. 2016:23:34.
doi: 10.1051/parasite/2016034. Epub 2016 Aug 26.

Distribution of Plasmodium species on the island of Grande Comore on the basis of DNA extracted from rapid diagnostic tests

Nasserdine Papa Mze  1 , Ambroise D Ahouidi  1 , Cyrille K Diedhiou  1 , Rahamatou Silai  2 , Mouhamadou Diallo  1 , Daouda Ndiaye  3 , Mbacké Sembene  4 , Souleymane Mboup  1
Affiliations

Distribution of Plasmodium species on the island of Grande Comore on the basis of DNA extracted from rapid diagnostic tests

Nasserdine Papa Mze et al. Parasite. 2016.

Abstract
in English, French

In the Union of Comoros, interventions for combating malaria have contributed to a spectacular decrease in the prevalence of the disease. We studied the current distribution of Plasmodium species on the island of Grande Comore using nested PCR. The rapid diagnostic tests (RDTs) currently used in the Comoros are able to identify Plasmodium falciparum but no other Plasmodium species. In this study, we tested 211 RDTs (158 positive and 53 negative). Among the 158 positive RDTs, 22 were positive for HRP2, 3 were positive only for pLDH, and 133 were positive for HRP2 and pLDH. DNA was extracted from a proximal part of the nitrocellulose membrane of RDTs. A total of 159 samples were positive by nested PCR. Of those, 156 (98.11%) were positive for P. falciparum, 2 (1.25%) were positive for P. vivaxI, and 1 (0.62%) was positive for P. malariae. None of the samples were positive for P. ovale. Our results show that P. falciparum is still the most dominant species on the island of Grande Comore, but P. vivax and P. malariae are present at a low prevalence.

Dans l’Union des Comores, des interventions pour la lutte contre le paludisme ont contribué à une diminution spectaculaire de la prévalence de la maladie. Nous avons étudié la répartition actuelle des espèces de Plasmodium sur l’île de Grande Comore par PCR imbriquée. Les tests de diagnostic rapide (TDRs) actuellement utilisés aux Comores sont en mesure d’identifier Plasmodium falciparum, mais pas d’autres espèces de Plasmodium. Dans cette étude, nous avons testé 211 TDRs (158 positifs et 53 négatifs). Parmi les 158 TDRs positifs, 22 étaient positifs pour HRP2, 3 étaient positifs seulement pour pLDH et 133 étaient positifs pour à HRP2 et pLDH. L’ADN a été extrait d’une partie proximale de la membrane de nitrocellulose des TDRs. Au total, 159 échantillons étaient positifs par PCR nichée. Parmi eux, 156 (98,11 %) étaient positifs pour P. falciparum, 2 (1,25 %) étaient positifs pour P. vivax et 1 (0,62 %) était positif pour P. malariae. Aucun des échantillons n’était positif pour P. ovale. Nos résultats montrent que P. falciparum est toujours l’espèce dominante dans l’île de Grande Comore, mais P. vivax et P. malariae sont présents à une faible prévalence.

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References

    1. Abba K, Deeks JJ, Olliaro P, Naing CM, Jackson SM, Takwoingi Y, Donegan S, Garner P. 2011. Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries. Cochrane Database of Systematic Reviews, CD008122. - PMC - PubMed
    1. Ayalew F, Tilahun B, Taye B. 2014. Performance evaluation of laboratory professionals on malaria microscopy in Hawassa Town, Southern Ethiopia. BioMed Central Research Notes, 7, 839. - PMC - PubMed
    1. Bartoloni A, Sabatinelli G, Benucci M. 1998. Performance of two rapid tests for Plasmodium falciparum malaria in patients with rheumatoid factors. New England Journal of Medicine, 338, 1075. - PubMed
    1. Bharti PK, Silawat N, Singh PP, Singh MP, Shukla M, Chand G, Dash AP, Singh N. 2008. The usefulness of a new rapid diagnostic test, the First Response Malaria Combo (pLDH/HRP2) card test, for malaria diagnosis in the forested belt of central. Malaria Journal, 7, 126. - PMC - PubMed
    1. Biswas S, Tomar D, Rao DN. 2005. Investigation of the kinetics of histidine-rich protein 2 and of the antibody responses to this antigen, in a group of malaria patients from India. Annals of Tropical Medicine and Parasitology, 99, 553–562. - PubMed

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