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. 2016 Oct 7;81(19):8796-8805.
doi: 10.1021/acs.joc.6b01439. Epub 2016 Sep 14.

HPLC-Assisted Automated Oligosaccharide Synthesis: Implementation of the Autosampler as a Mode of the Reagent Delivery

Salvatore G Pistorio  1 , Swati S Nigudkar  1 , Keith J Stine  1 , Alexei V Demchenko  1
Affiliations

HPLC-Assisted Automated Oligosaccharide Synthesis: Implementation of the Autosampler as a Mode of the Reagent Delivery

Salvatore G Pistorio et al. J Org Chem. .

Abstract

The development of a useful methodology for simple, scalable, and transformative automation of oligosaccharide synthesis that easily interfaces with existing methods is reported. The automated synthesis can now be performed using accessible equipment where the reactants and reagents are delivered by the pump or the autosampler and the reactions can be monitored by the UV detector. The HPLC-based platform for automation is easy to setup and adapt to different systems and targets.

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Conflict of interest statement

Notes

The authors declare no competing financial interest.

Figures

Scheme 1
Scheme 1
Original Set-up for HPLC-Assisted Synthesis.
Scheme 2
Scheme 2
Synthesis of the Solid-Phase-Bound Acceptor 3
Scheme 3
Scheme 3
Refinement of the Glycosylation-Cleavage Sequence for the Synthesis of Disaccharide 11
Scheme 4
Scheme 4
Automation of Glycosylation-Deprotection-Cleavage Sequences for the Synthesis of Oligosaccharides 12 and 14
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References

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