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. 2016 Oct;31(5):406-414.
doi: 10.1007/s12250-016-3808-3. Epub 2016 Aug 29.

Ficolin-2 binds to HIV-1 gp120 and blocks viral infection

Fengling Luo  1 , Tielong Chen  1   2 , Jun Liu  1 , Xihui Shen  3 , Yinnan Zhao  1 , Rongge Yang  4 , Xiaolian Zhang  5
Affiliations

Ficolin-2 binds to HIV-1 gp120 and blocks viral infection

Fengling Luo et al. Virol Sin. 2016 Oct.

Abstract

Ficolin-2 is a lectin complement pathway activator present in normal human plasma and usually associated with infectious diseases, but little is known about the role of ficolin-2 in human immunodeficiency virus (HIV) infection. Here, we describe our novel findings that serum ficolin-2 concentrations of 103 HIV-1 patients were much higher compared to those of 57 healthy donors. In vitro analysis showed that HIV-1 infection could enhance ficolin-2 expression. We further demonstrated that recombinant ficolin-2 protein could bind with HIV-1 envelope glycoprotein gp120, and subsequently induce complement dependent cytotoxicity. Moreover, ficolin-2 could block the entry of HIV-1 into target cells (TZM-b1 and MT-2 cells) and infection in a ficolin-2 dosedependent manner. To our knowledge, this is the first report about the protective role of ficolin-2 against HIV-1 infection and our study suggests that ficolin-2 is an important human innate immune molecule against HIV.

Keywords: HIV-1; ficolin-2; gp120.

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