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. 2016:1455:203-11.
doi: 10.1007/978-1-4939-3792-9_15.

Fluorescence-Activated Nucleolus Sorting in Arabidopsis

Frédéric Pontvianne  1   2 , Myriam Boyer-Clavel  3   4 , Julio Sáez-Vásquez  5   6
Affiliations

Fluorescence-Activated Nucleolus Sorting in Arabidopsis

Frédéric Pontvianne et al. Methods Mol Biol. 2016.

Abstract

Nucleolar isolation allows exhaustive characterization of the nucleolar content. Centrifugation-based protocols are not adapted to isolation of nucleoli directly from a plant tissue because of copurification of cellular debris. We describe here a method that allows the purification of nucleoli using fluorescent-activated cell sorting from Arabidopsis thaliana leaves. This approach requires the expression of a specific nucleolar protein such as fibrillarin fused to green fluorescent protein in planta.

Keywords: Arabidopsis thaliana; FACS; Fibrillarin; Nucleolus.

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