doi: 10.1007/978-1-4939-3792-9_15.
Fluorescence-Activated Nucleolus Sorting in Arabidopsis
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- PMID: 27576720
- DOI: 10.1007/978-1-4939-3792-9_15
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Fluorescence-Activated Nucleolus Sorting in Arabidopsis
Methods Mol Biol.
2016.
Abstract
Nucleolar isolation allows exhaustive characterization of the nucleolar content. Centrifugation-based protocols are not adapted to isolation of nucleoli directly from a plant tissue because of copurification of cellular debris. We describe here a method that allows the purification of nucleoli using fluorescent-activated cell sorting from Arabidopsis thaliana leaves. This approach requires the expression of a specific nucleolar protein such as fibrillarin fused to green fluorescent protein in planta.
Keywords: Arabidopsis thaliana; FACS; Fibrillarin; Nucleolus.
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