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Review
. 2017 Feb:43:34-40.
doi: 10.1016/j.copbio.2016.08.001. Epub 2016 Aug 28.

The future of NMR-based metabolomics

John L Markley  1 , Rafael Brüschweiler  2 , Arthur S Edison  3 , Hamid R Eghbalnia  4 , Robert Powers  5 , Daniel Raftery  6 , David S Wishart  7
Affiliations
Review

The future of NMR-based metabolomics

John L Markley et al. Curr Opin Biotechnol. 2017 Feb.

Abstract

The two leading analytical approaches to metabolomics are mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. Although currently overshadowed by MS in terms of numbers of compounds resolved, NMR spectroscopy offers advantages both on its own and coupled with MS. NMR data are highly reproducible and quantitative over a wide dynamic range and are unmatched for determining structures of unknowns. NMR is adept at tracing metabolic pathways and fluxes using isotope labels. Moreover, NMR is non-destructive and can be utilized in vivo. NMR results have a proven track record of translating in vitro findings to in vivo clinical applications.

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Figures

Figure 1
Figure 1
Schematic representation of metabolic “dark matter”.
See this image and copyright information in PMC

References

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