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. 2016 Sep;101(9):e378-81.
doi: 10.3324/haematol.2016.141929.

Complementarity determining region-independent recognition of a superantigen by B-cell antigen receptors of mantle cell lymphoma

Michael Fichtner  1 , Elmar Spies  1 , Henning Seismann  1 , Kristoffer Riecken  2 , Niklas Engels  3 , Barbara Gösch  1 , Judith Dierlamm  1 , Helwe Gerull  4 , Peter Nollau  4 , Wolfram Klapper  5 , Martin Dreyling  6 , Mascha Binder  7 , Martin Trepel  8
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Complementarity determining region-independent recognition of a superantigen by B-cell antigen receptors of mantle cell lymphoma

Michael Fichtner et al. Haematologica. 2016 Sep.
No abstract available

Keywords: B-cell receptor; SpA; mantle cell lymphoma; protein A; superantigen.

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Figures

Figure 1.
Figure 1.
IGHV3 expressing MCL BCRs bind to the staphylococcal protein A leading to BCR pathway activation. (A) Amino acid sequence alignment of the Staphylococcus aureus protein A (SpA) binding motif of all IGHV3-containing MCL-BCR- as well as some CLL-BCR- and FL-BCR-derived immunoglobulins. The motif is indicated in the top row. Deviations are highlighted with black background. (B) ELISA with different Fab immunoglobulin fragments derived from MCL, CLL and FL samples to determine their reactivity to SpA. Note that FL10 harbored a highly mutated SpA-motif and is completely inert. (C) Ca2+ flux analysis of Ramos cells expressing MCL-derived membrane-bound immunoglobulins (mIgs). Transduced cells were BCR-stimulated by adding either SpA (bottom row) or an anti-human-Fab antibody (top row). The time of addition is indicated by the gaps in the plots. Non-transduced cells served as control.
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