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. 2016 Aug 1;20(3):107-11.
doi: 10.5935/1518-0557.20160025.

A descriptive study of culture media in Brazilian assisted reproduction clinics

Ana Bartmann  1   2 , Amanda Turato Barbosa do Amaral  3 , Letícia Gonçalves  1   2
Affiliations

A descriptive study of culture media in Brazilian assisted reproduction clinics

Ana Bartmann et al. JBRA Assist Reprod. .

Abstract

Objective: The present study aimed to draw a profile of the most commonly used media and protocol characteristics from assisted reproduction technology (ART) facilities in Brazil.

Methods: To obtain an overview of ART methods and culture media, a questionnaire was given to embryologists from ART clinics in Brazil. Further research in scientific papers and journals was carried out for describing the processes around Brazil, USA and Europe.

Results: From the questionnaire, we found that the embryo medium mostly used is CSCMTM from Irvine Scientific, represented 37.04% in Brazilian ART clinics; interestingly, 70.37% of clinics exchange the embryo media bath; however, 70.37% do not change the media type. Transfers in Brazilian clinics were variable, but day 3 transfer was a procedure seen in 37.04%. The remaining embryos are habitually maintained in prolonged cultivation in 51.85% of the clinics interviewed.

Conclusion: Although there are numerous studies trying to better understand embryo culture media influences, there is a lack of evidence for choosing one as the most appropriate. In short, it is a random decision for such an essential stage of In Vitro Fertilization.

Keywords: Brazilian clinics; Culture media; Embryo transfer.

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Conflict of interest statement

CONFLICT OF INTERESTS No conflict of interest have been declared.

Comment in

References

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