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Meta-Analysis
. 2016 Aug 31;57(4):351-62.
doi: 10.3325/cmj.2016.57.351.

Angiotensinogen-M235T as a risk factor for myocardial infarction in Asian populations: a genetic association study and a bioinformatics approach

Fariba Raygan, Mohammad Karimian  1 , Atefeh Rezaeian, Bahareh Bahmani, Mohaddeseh Behjati
Affiliations
Meta-Analysis

Angiotensinogen-M235T as a risk factor for myocardial infarction in Asian populations: a genetic association study and a bioinformatics approach

Fariba Raygan et al. Croat Med J. .

Abstract

Aim: To investigate if there is an association between M235T polymorphism of angiotensinogen gene and myocardial infarction (MI) risk and perform a meta-analysis and an in silico approach.

Methods: This case-control study included 340 participants (155 MI patients and 185 controls) examined at Kashan University of Medical Sciences (Kashan, Iran) between 2013 and 2015. Meta-analysis included 25 studies with 6334 MI patients and 6711 controls. Bioinformatics tools were applied to evaluate the impact of M235T polymorphism on angiotensinogen function and structure.

Results: Genetic association study revealed a significant association between TT genotype (odds ratio [OR] 2.08, 95% confidence interval [CI] 1.08-4.00, P=0.029) and T allele (OR 1.45, 95% CI 1.06-1.99, P=0.021) and MI risk. Meta-analysis also revealed a significant association between M235T polymorphism and MI risk in allelic (OR 1.55, 95% CI 1.10-2.18, P=0.012) and recessive (OR 1.69, 95% CI 1.13-2.53, P=0.010) models within Asian population. In silico-analysis revealed that M235T fundamentally changed the function of angiotensinogen (score 32; expected accuracy 66%).

Conclusions: Our study suggests that M235T polymorphism might be a helpful biomarker for screening of susceptible individuals for MI in Asian population.

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Figures

Figure 1
Figure 1
Flowchart of the study selection process.
Figure 2
Figure 2
Forest plot for the association of M235T polymorphism of angiotensinogen gene (AGT-M235T) with myocardial infarction (MI).
Figure 3
Figure 3
Funnel plot for association of M235T polymorphism of angiotensinogen gene (AGT-M235T) with myocardial infarction (MI) in Asian population.
Figure 4
Figure 4
M235T substitution and angiotensinogen (AGT) secondary structure. Representation of Met and Thr residues in position 235 (A&A’). Secondary structure of AGT for 235M and 235T phenotypes in position 268 (B & B’).
Figure 5
Figure 5
Hydrophobicity, PredictProtein, and SNAP predictions. The hydrophobicity plot for 235M and 235T phenotypes (A&A’). The PredictProtein and SNAP plots for M235T substitution, respectively (B’&B).
See this image and copyright information in PMC

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