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. 2017 Jan;199(1):177-183.
doi: 10.1007/s00203-016-1285-6. Epub 2016 Sep 2.

Genome-based insights into the resistome and mobilome of multidrug-resistant Aeromonas sp. ARM81 isolated from wastewater

Marcin Adamczuk  1 , Lukasz Dziewit  2
Affiliations

Genome-based insights into the resistome and mobilome of multidrug-resistant Aeromonas sp. ARM81 isolated from wastewater

Marcin Adamczuk et al. Arch Microbiol. 2017 Jan.

Abstract

The draft genome of multidrug-resistant Aeromonas sp. ARM81 isolated from a wastewater treatment plant in Warsaw (Poland) was obtained. Sequence analysis revealed multiple genes conferring resistance to aminoglycosides, β-lactams or tetracycline. Three different β-lactamase genes were identified, including an extended-spectrum β-lactamase gene bla PER-1. The antibiotic susceptibility was experimentally tested. Genome sequencing also allowed us to investigate the plasmidome and transposable mobilome of ARM81. Four plasmids, of which two carry phenotypic modules (i.e., genes encoding a zinc transporter ZitB and a putative glucosyltransferase), and 28 putative transposase genes were identified. The mobility of three insertion sequences (isoforms of previously identified elements ISAs12, ISKpn9 and ISAs26) was confirmed using trap plasmids.

Keywords: Aeromonas sp. ARM81; Antibiotic resistance gene; Genome; Plasmid; Transposable element.

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Figures

Fig. 1
Fig. 1
Linear maps showing the genetic structure of the Tn5393k transposon identified in Aeromonas sp. ARM81 and Tn5393d of A. faecalis FL-424/98 (NCBI accession number: AJ627643). Arrows indicate predicted genes and their transcriptional orientation. The gray-shaded areas connect DNA regions with 100 % nucleotide sequence identity. The following open reading frames are indicated: tnpA transpose-encoding gene, tnpR resolvase-encoding gene, aph(3′)-VIb, aph(3′′)-Ib and aph(6)-Id aminoglycoside resistance genes, and bla PER-1 β-lactamase-encoding gene
Fig. 2
Fig. 2
Linear maps showing the genetic structure of the circular plasmids pARM81P1-P4 of Aeromonas sp. ARM81 and the related plasmids of Aeromonas spp. Arrows indicate predicted genes and their transcriptional orientation. The gray-shaded areas connect DNA regions of different plasmids with at least 66 % nucleotide sequence identity. The following open reading frames are indicated: repA replication protein A-encoding gene, repB replication protein B-encoding gene, mobA mobilization protein A (relaxase)-encoding gene, mobC mobilization protein C-encoding gene, zitB zinc transporter ZitB-encoding gene, arsR ArsR-family transcriptional regulator-encoding gene, tox toxin (of a toxin–antitoxin system)-encoding gene, ant antitoxin (of a toxin–antitoxin system)-encoding gene, gtr glucosyltransferase-encoding gene and rep/virE primase/virulence-associated protein-encoding gene
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