doi: 10.1016/0379-0738(89)90205-3.
A method of human diaphorase locus 3 phenotyping in human semen by isoelectric focusing
Affiliations
- PMID: 2759518
- DOI: 10.1016/0379-0738(89)90205-3
Item in Clipboard
A method of human diaphorase locus 3 phenotyping in human semen by isoelectric focusing
Forensic Sci Int.
1989 Jul.
Abstract
A rapid method for the separation of diaphorase (DIA 3) isozymes in the pH range 6-8 is presented. Isoelectric focusing (IEF) of DIA 3 on 0.2 mm thick polyacrylamide gels took 60 min at 5 degrees C. Separated bands are sharp and common phenotypes are readily distinguishable. The method combines high resolution, speed, reproducibility and reagent economy with simplicity.
Similar articles
-
A new electrophoretically distinguishable variant of human diaphorase locus 3(DIA3):DIA3 6-1.Hum Genet. 1990 Jan;84(2):213. doi: 10.1007/BF00208946. Hum Genet. 1990. PMID: 2298459
-
A new allele at the human diaphorase DIA3 locus: DIA5(3).Hum Genet. 1986 Apr;72(4):354-5. doi: 10.1007/BF00290965. Hum Genet. 1986. PMID: 3754538
-
DIA3 phenotyping in human semen and seminal stains by isoelectric focusing.Z Rechtsmed. 1987;98(3):207-12. doi: 10.1007/BF00200343. Z Rechtsmed. 1987. PMID: 3630414
-
Typing study of human semen DIA3 by isoelectric focusing--distribution in the Wuhan population, China.Forensic Sci Int. 1990 Feb;44(2-3):203-7. doi: 10.1016/0379-0738(90)90251-s. Forensic Sci Int. 1990. PMID: 2318475
-
Electrophoretic phenotyping of erythrocyte enzymes.J Chromatogr. 1991 Sep 13;569(1-2):297-321. doi: 10.1016/0378-4347(91)80235-5. J Chromatogr. 1991. PMID: 1834688 Review.
Cited by
-
A new electrophoretically distinguishable variant of human diaphorase locus 3(DIA3):DIA3 6-1.Hum Genet. 1990 Jan;84(2):213. doi: 10.1007/BF00208946. Hum Genet. 1990. PMID: 2298459
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
