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. 1989 Jul;42(1-2):131-4.
doi: 10.1016/0379-0738(89)90205-3.

A method of human diaphorase locus 3 phenotyping in human semen by isoelectric focusing

R Pawlowski  1
Affiliations

A method of human diaphorase locus 3 phenotyping in human semen by isoelectric focusing

R Pawlowski. Forensic Sci Int. 1989 Jul.

Abstract

A rapid method for the separation of diaphorase (DIA 3) isozymes in the pH range 6-8 is presented. Isoelectric focusing (IEF) of DIA 3 on 0.2 mm thick polyacrylamide gels took 60 min at 5 degrees C. Separated bands are sharp and common phenotypes are readily distinguishable. The method combines high resolution, speed, reproducibility and reagent economy with simplicity.

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