Optimization of murine model for Besnoitia caprae

Abstract

It has been shown that mice, particularly the BALB/c ones, are susceptible to infection by some of the apicomplexan parasites. To compare the susceptibility of the inbred BALB/c, outbred BALB/c and C57 BL/6 to Besnoitia caprae inoculation and to determine LD50, 30 male inbred BALB/c, 30 outbred BALB/c and 30 C57 BL/6 mice were assigned into 18 groups of 5 mice. Each group was inoculated intraperitoneally with 12.5 × 10(3), 25 × 10(3), 5 × 10(4), 1 × 10(5), 2 × 10(5) tachyzoites and a control inoculum of DMEM, respectively. The inbred BALB/c was found the most susceptible strain among the experienced mice strains so the LD50 per inbred BALB/c mouse was calculated as 12.5 × 10(3.6) tachyzoites while the LD50 for the outbred BALB/c and C57 BL/6 was 25 × 10(3.4) and 5 × 10(4) tachyzoites per mouse, respectively. To investigate the impact of different routes of inoculation in the most susceptible mice strain, another seventy five male inbred BALB/c mice were inoculated with 2 × 10(5) tachyzoites of B. caprae via various inoculation routes including: subcutaneous, intramuscular, intraperitoneal, infraorbital and oral. All the mice in the oral and infraorbital groups survived for 60 days, whereas the IM group showed quicker death and more severe pathologic lesions, which was then followed by SC and IP groups. Therefore, BALB/c mouse is a proper laboratory model and IM inoculation is an ideal method in besnoitiosis induction and a candidate in treatment, prevention and testing the efficacy of vaccines for besnoitiosis.

Keywords: Besnoitia caprae; Murine model; Susceptibility.

Fig. 1

Histopathology section from the kidneys of a mouse infected with 1 × 10⁵ tachyzoites. a. sever tubular degeneration (H & E, ×240) and b. Presence of congestion (thin arrow), and mononuclear cells infiltration (thick arrow) (H & E, ×200)

Fig. 2

Histopathology section from the liver of a mouse infected with 5 × 10⁴ tachyzoites intramuscularly. Severe fatty change (thin arrows) in association with congestion (thick arrows) (H & E, ×350)

Fig. 3

Histopathology section from the lungs of a mouse infected with 5 × 10⁴ tachyzoitestachyzoites. a. congestion (H & E, ×110), b. Thickening of alveolar septa and infiltration of inflammatory cells (H & E, ×200)