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. 2016 Sep;40(3):901-5.
doi: 10.1007/s12639-014-0601-9. Epub 2014 Oct 30.

Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt

Marwa M I Ghallab  1 , Inas Z Abdel Aziz  1 , Eman Y Shoeib  1 , Ayman A El-Badry  1
Affiliations

Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt

Marwa M I Ghallab et al. J Parasit Dis. 2016 Sep.

Abstract

Cryptosporidium is a significant cause of diarrhea worldwide especially in children. Infection may end fatally in immunocompromised patients. Multi-attribute analysis was used to determine the lab utility of 4 diagnostics; coproscopy of AF stained fecal smear, fecal immunoassays by ICT and ELISA and copro-nPCR assay targeting Hsp90 gene, for detection of Cryptosporidium in stool of 250 Egyptian children (150 diarrheic and 100 non-diarrhaeic children). Also, to determine Cryptosporidium molecular prevalence. Cryptosporidium was an important enteric pathogen among both diarrheic and non-diarrheic study children with a clearly high prevalence of 16.4 % (n = 41). Conventional methods had perfect specificity (100 %) but couldn`t be used as a consistent single detection method due to their lowered sensitivities. Multi-attribute analysis ranked nPCR the highest test for lab use. Being the test with the best diagnostic yield, nPCR is a reliable diagnostic test and is going to replace conventional methods for reliable detection of Cryptosporidium.

Keywords: AF stain; Copro-DNA; Cryptosporidium; ELISA; Fecal immunoassay; Hsp90; ICT; Multi-attribute; Nested PCR.

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Figures

Fig. 1
Fig. 1
Agarose gel electrophoresis for the products of the nPCR targeting Hsp90 gene of Cryptosporidium at 676–685 bp. L 100 bp DNA molecular weight marker. Lane 1 positive control. Lanes 2 negative control. Lanes 3–7 positive samples
See this image and copyright information in PMC

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