Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Sep;40(3):1066-70.
doi: 10.1007/s12639-014-0635-z. Epub 2014 Dec 20.

Immune response of newborn BALB/c mice to Cryptosporidium infection

Nasser Ahmadian  1 , Roghiyeh Pashaei-Asl  2 , Masomeh Ahmadian  3 , Mohammad Rahmati-Yamchi  4 , Saed Shahabi  1 , Hossein Vazini  5
Affiliations

Immune response of newborn BALB/c mice to Cryptosporidium infection

Nasser Ahmadian et al. J Parasit Dis. 2016 Sep.

Abstract

Cryptosporidium parvum is a protozoan parasite which causes diarrheal in human and animals worldwide. Infection transmission has reported through oral-fecal by infectious objects through foods and drinks. In this study we explored the immune response pathway in animal model for C. parvum to develop the new treatment way. Oocysts collected from fecal positive for C. parvum and diluted about 1:5 in sucrose solution. New born BALB/c mice (3 days) divided to 2 different groups. Control group hadn't received any oocyst, the test groups received 5 × 10(5) oocysts. 5 mice selected for each control group and 11 mice chosen for each test group. Blood collected from heart bleeds in days of 6, 9, 12 and 16. Protein concentrations determined by bio-photometer. Dot blotting used to find out total antibody concentrations oocyst antigen. Among the test and the control groups, blots appeared in test group which means antibody production, but not any blot observed in the control groups. The non-characteristic proteins in serum were measured by the biophotometer. In this study, we investigated antibody serum production against C. parvum oocysts in new born BALB/c mice. The detected antibody through dot blot technique was our aims which had conjugated to our characteristic antiserum. The recorded numbers for the controls by biophotometer related to the non characteristic proteins in serum. The results of this study can used to produce polyclonal or monoclonal antibodies against cryptosporidiosis.

Keywords: BALB/c mice; Cryptosporidium parvum; Immune response.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Protein concentration (mg/ml) for test groups after 6, 9, 12 and 16 days. PC protein concentration
Fig. 2
Fig. 2
Dot blotting assay for determination of sensitivity of the antigen a protein extracted from mice after 6 days, b1 after 9 days, b2 after 12 days, c after 16 days of infection. Antigen was applied as 3 µl dots on a nitrocellulose strip
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Angus KW. Cryptosporidiosis and AIDS. Baillieres Clin Gastroenterol. 1990;4(2):425–441. doi: 10.1016/0950-3528(90)90010-E. - DOI - PubMed
    1. Arrowood MJ, Sterling CR. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J Parasitol. 1987;73(2):314–319. doi: 10.2307/3282084. - DOI - PubMed
    1. Borad A, Ward H. Human immune responses in cryptosporidiosis. Future Microbiol. 2010;5:507–519. doi: 10.2217/fmb.09.128. - DOI - PMC - PubMed
    1. Bouzid M, Hunter PR, Chalmers RM, Tyler KM. Cryptosporidium pathogenicity and virulence. Clin Microbiol Rev. 2013;26(1):115–134. doi: 10.1128/CMR.00076-12. - DOI - PMC - PubMed
    1. Chen XM, O’Hara SP, Nelson JB, Splinter PL, Small AJ, Tietz PS, et al. Multiple TLRs are expressed in human cholangiocytes and mediate host epithelial defence responses to Cryptosporidium parvum via activation of NF-B. J Immunol. 2005;175(11):7447–7456. doi: 10.4049/jimmunol.175.11.7447. - DOI - PubMed

LinkOut - more resources