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. 2016 Jul 29;4(8):apps.1600021.
doi: 10.3732/apps.1600021. eCollection 2016 Aug.

New development and validation of 50 SSR markers in breadfruit (Artocarpus altilis, Moraceae) by next-generation sequencing

Fabien De Bellis  1 , Roger Malapa  2 , Valérie Kagy  3 , Stéphane Lebegin  3 , Claire Billot  1 , Jean-Pierre Labouisse  1
Affiliations

New development and validation of 50 SSR markers in breadfruit (Artocarpus altilis, Moraceae) by next-generation sequencing

Fabien De Bellis et al. Appl Plant Sci. .

Abstract

Premise of the study: Using next-generation sequencing technology, new microsatellite loci were characterized in Artocarpus altilis (Moraceae) and two congeners to increase the number of available markers for genotyping breadfruit cultivars.

Methods and results: A total of 47,607 simple sequence repeat loci were obtained by sequencing a library of breadfruit genomic DNA with an Illumina MiSeq system. Among them, 50 single-locus markers were selected and assessed using 41 samples (39 A. altilis, one A. camansi, and one A. heterophyllus). All loci were polymorphic in A. altilis, 44 in A. camansi, and 21 in A. heterophyllus. The number of alleles per locus ranged from two to 19.

Conclusions: The new markers will be useful for assessing the identity and genetic diversity of breadfruit cultivars on a small geographical scale, gaining a better understanding of farmer management practices, and will help to optimize breadfruit genebank management.

Keywords: Artocarpus altilis; Illumina; Moraceae; breadfruit; high-throughput sequencing; microsatellites.

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