Biological function of unique sulfated glycosaminoglycans in primitive chordates

Abstract

Glycosaminoglycans with unique sulfation patterns have been identified in different species of ascidians (sea squirts), a group of marine invertebrates of the Phylum Chordata, sub-phylum Tunicata (or Urochordata). Oversulfated dermatan sulfate composed of [4-α-L-IdoA-(2-O-SO₃)^-1 → 3-β-D-GalNAc(4-OSO₃)^-1]_n repeating disaccharide units is found in the extracellular matrix of several organs, where it seems to interact with collagen fibers. This dermatan sulfate co-localizes with a decorin-like protein, as indicated by immunohistochemical analysis. Low sulfated heparin/heparan sulfate-like glycans composed mainly of [4-α-L-IdoA-(2-OSO₃)^-1 → 4-α-D-GlcN(SO₃)^-1 (6-O-SO₃)^-1]_n and [4-α-L-IdoA-(2-O-SO₃)^-1 → 4-α-D-GlcN(SO₃)^-1]_n have also been described in ascidians. These heparin-like glycans occur in intracellular granules of oocyte assessory cells, named test cells, in circulating basophil-like cells in the hemolymph, and at the basement membrane of different ascidian organs. In this review, we present an overview of the structure, distribution, extracellular and intracellular localization of the sulfated glycosaminoglycans in different species and tissues of ascidians. Considering the phylogenetic position of the subphylum Tunicata in the phylum Chordata, a careful analysis of these data can reveal important information about how these glycans evolved from invertebrate to vertebrate animals.

Keywords: Ascidians; Dermatan sulfate; Glycosaminoglycans; Heparin/heparan sulfate; Invertebrates; Primitive chordates.