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. 1989 Jul 11;17(13):5115-23.
doi: 10.1093/nar/17.13.5115.

Chemiluminescent detection of DNA: application for DNA sequencing and hybridization

S Beck  1 , T O'Keeffe, J M Coull, H Köster
Affiliations
Free PMC article

Chemiluminescent detection of DNA: application for DNA sequencing and hybridization

S Beck et al. Nucleic Acids Res. .
Free PMC article

Abstract

A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a chemiluminescent reaction. The image is captured within seconds by a Polaroid or X-ray film. The method is capable of detecting DNA in the hundred attomol range.

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References

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